This recombinant Glutathione S-transferase (GST) protein is encoded by the parasitic Schistosoma japonicum GST gene. The parasite GST protein shows ~60% homogy with class Mu isozymes of mammalian GST (EC 22.214.171.124). The mammalian GST super family consists of four classes: Alpha, Mu, Pi, and Theta. GST catalyzes the conjugation of reduced glutathione to electrophiles on many substrates, thus detoxifying endogenous compounds such as lipid peroxides.
Many recombinant proteins are expressed as glutathione S-transferase fusion proteins to facilitate purification. The recombinant GST can be used as a negative control for recombinant growth factors expressed as GST fusion proteins. The C-terminal end of the protein harbors an engineered thrombin cleavage site and a protein kinase A (PKA) phosphorylation site. The protein can therefore be radioactively labeled with 32P-g-ATP and PKA.
From the laboratory of Te-Chung Lee, PhD, University at Buffalo.
Part of The Investigator's Annexe program.
|Name:||Glutathione S-transferase (GST)|
|Source:||Recombinantly expressed in E. coliBL21|
|Molecular Weight:||25 kDa|
|Amino Acid Sequence:||MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK|
Detection of GST by SDS-PAGE
Purified GST and GST-YAF2 proteins (2 ug each) were fractionated by 12% SDS-PAGE and stained by Coomassie Blue.