Tetrazine-DBCO
Tetrazine-DBCO is a reagent for converting azido-modified peptides or biopolymers into tetrazine-modified peptides or biopolymers.
Highlights:
- Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
- Chemoselective – tetzines and trans-cyclooctene groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
- Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available
- Solubility – easily dissolves in aqueous buffers
Tetrazine-DBCO is a water-soluble, heterobifunctional reagent that enables simple and efficient conversion of azide-modified peptides into tetrazine-tagged peptides via Cu(I)-free click reaction. Once a peptide or biopolymer is tetrazine-labeled, it can be efficiently ligated to a TCO-labeled molecule to produce a stable conjugate.
Tetrazine-DBCO is a reagent for converting azido-modified peptides or biopolymers into tetrazine-modified peptides or biopolymers.
Highlights:
- Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
- Chemoselective – tetzines and trans-cyclooctene groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
- Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available
- Solubility – easily dissolves in aqueous buffers
Tetrazine-DBCO is a water-soluble, heterobifunctional reagent that enables simple and efficient conversion of azide-modified peptides into tetrazine-tagged peptides via Cu(I)-free click reaction. Once a peptide or biopolymer is tetrazine-labeled, it can be efficiently ligated to a TCO-labeled molecule to produce a stable conjugate.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Small Molecule |
| Name: | Tetrazine?DBCO |
| Chemical Formula: | C34H33N7O7S (protonated) |
| Molecular Weight: | 683.77 (protonated), 784.92 (triethylammonium salt) |
| Variant MPN: | 1022 |
| Purity: | >95%, HPLC |
| Solubility: | Water, DMSO, DMF, MeOH |
| Storage: | -20C.Desiccate |
| Shipped: | Ambient |
Documentation
Comments
Applications: Protein-protein conjugation, protein-peptide conjugation, protein-small molecule conjugation, 18F radiolabeling, protein-oligonucleotide conjugation, surface modification
References
- Karver, M. R., et. al. (2012). "Bioorthogonal Reaction Pairs Enable Simultaneous, Selective, Multi-Target Imaging." Angew. Chem. Int. Ed., 51:920-922.
- Blackman, M. L., et. al. (2008). "Tetrazine Ligation: Fast Bioconjugation Based on Inverse- Electron-Demand Diels-Alder Reactivity." J. Am. Chem. Soc., 130:13518-13519
- Devaraj, N. K., et. al. (2008) "Tetrazine-Based Cycloadditions: Application to Pretargeted Live Cell Imaging." Bioconjugate Chem., 19(12): 22972299.
- Devaraj, N. K., et. al. (2009) "Fast and Sensitive Pre-Targeted Labeling of Cancer Cells through a Tetrazine/trans-Cyclooctene Cycloaddition." Angew. Chem. Int. Ed., DOI: 10.1002/anie.200903233
- Haun, J.B., et. al. (2009) " Probing Intracellular Biomarkers and Mediators of Cell Activation Using Nanosensor and Bioorthogonal Chemistry" ACS Nano., 5:3204-321
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