Tetrazine-DBCO is a reagent for converting azido-modified peptides or biopolymers into tetrazine-modified peptides or biopolymers.


  • Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
  • Chemoselective – tetzines and trans-cyclooctene groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
  • Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available
  • Solubility – easily dissolves in aqueous buffers

Tetrazine-DBCO is a water-soluble, heterobifunctional reagent that enables simple and efficient conversion of azide-modified peptides into tetrazine-tagged peptides via Cu(I)-free click reaction. Once a peptide or biopolymer is tetrazine-labeled, it can be efficiently ligated to a TCO-labeled molecule to produce a stable conjugate.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Tetrazine-DBCO, 10 mg
10mg In stock
Regular Price:$176.00
On Sale:
Tetrazine-DBCO, 25 mg
25mg In stock
Regular Price:$464.00
On Sale:
Tetrazine-DBCO, 100 mg
100mg In stock
Regular Price:$1,270.00
On Sale:

Product Type: Small Molecule
Name: Tetrazine?DBCO
Chemical Formula: C34H33N7O7S (protonated)
Molecular Weight: 683.77 (protonated), 784.92 (triethylammonium salt)
Variant MPN: 1022
Purity: >95%, HPLC
Solubility: Water, DMSO, DMF, MeOH
Storage: -20C.Desiccate
Shipped: Ambient


Applications: Protein-protein conjugation, protein-peptide conjugation, protein-small molecule conjugation, 18F radiolabeling, protein-oligonucleotide conjugation, surface modification

  1. Karver, M. R., et. al. (2012). "Bioorthogonal Reaction Pairs Enable Simultaneous, Selective, Multi-Target Imaging." Angew. Chem. Int. Ed., 51:920-922.
  2. Blackman, M. L., et. al. (2008). "Tetrazine Ligation: Fast Bioconjugation Based on Inverse- Electron-Demand Diels-Alder Reactivity." J. Am. Chem. Soc., 130:13518-13519
  3. Devaraj, N. K., et. al. (2008) "Tetrazine-Based Cycloadditions: Application to Pretargeted Live Cell Imaging." Bioconjugate Chem., 19(12): 2297–2299.
  4. Devaraj, N. K., et. al. (2009) "Fast and Sensitive Pre-Targeted Labeling of Cancer Cells through a Tetrazine/trans-Cyclooctene Cycloaddition." Angew. Chem. Int. Ed., DOI: 10.1002/anie.200903233
  5. Haun, J.B., et. al. (2009) " Probing Intracellular Biomarkers and Mediators of Cell Activation Using Nanosensor and Bioorthogonal Chemistry" ACS Nano., 5:3204-321

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