Methyltetrazine-PEG4-Alkyne is a reagent for converting azido-modified peptides or biopolymers into tetrazine-modified peptides or biopolymers.


  • Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
  • Chemoselective – tetzines and trans-cyclooctene groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
  • Unprecedented kinetics – inverse-electron demand Diels-Alder chemistry is the fastest bioorthogonal ligation available, delivering conjugates within 30 min at 1 mg/mL concentrations
  • Extended PEG4 Spacer – reduces aggregation, minimizes steric hindrance, and enhances solubility

Methyltetrazine-PEG4-Alkyne is a heterobifunctional reagent that enables simple and efficient conversion of azide-modified peptides into tetrazine-tagged peptides for subsequent ligation to tetrazine-labeled biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary TCO-containing molecules.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Methyltetrazine-PEG4-Alkyne, 10 mg
10mg In stock
Regular Price:$153.00
On Sale:
Methyltetrazine-PEG4-Alkyne, 25 mg
25mg In stock
Regular Price:$282.00
On Sale:
Methyltetrazine-PEG4-Alkyne, 100 mg
100mg In stock
Regular Price:$701.00
On Sale:

Product Type: Small Molecule
Name: Methyltetrazine-PEG4-Alkyne
Chemical Formula: C20H26N4O5
Molecular Weight: 402.44
Variant MPN: 1013
Purity: >95%, HPLC
Solubility: DCM, Chloroform, DMSO, DMF
Storage: -20C.Desiccate
Shipped: Ambient


Applications: Protein-protein conjugation, protein-antibody conjugation, protein-small molecule conjugation, 18F radiolabeling, protein-oligonucleotide conjugation, surface modification

  1. Blackman, M. L., et. al. (2008). "Tetrazine Ligation: Fast Bioconjugation Based on Inverse- Electron-Demand Diels-Alder Reactivity." J. Am. Chem. Soc., 130:13518-13519
  2. Devaraj, N. K., et. al. (2008) "Tetrazine-Based Cycloadditions: Application to Pretargeted Live Cell Imaging." Bioconjugate Chem., 19(12): 2297–2299.
  3. Devaraj, N. K., et. al. (2009) "Fast and Sensitive Pre-Targeted Labeling of Cancer Cells through a Tetrazine/trans-Cyclooctene Cycloaddition." Angew. Chem. Int. Ed., DOI: 10.1002/anie.200903233
  4. Haun, J.B., et. al. (2009) " Probing Intracellular Biomarkers and Mediators of Cell Activation Using Nanosensor and Bioorthogonal Chemistry" ACS Nano., 5:3204-321

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