Cre (PGK) Lentivirus

This premade lentivirus includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction and expression of the target genes as well as antibiotics resistant genes or reporters. A woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is believed to promote RNA processing events and nuclear export, boosting expression of your gene of interest in target cells by facilitating the production of mature mRNA from transcripts initiated by the virus's internal promoter (e.g. pCMV).

Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (loxP sites).

Catalog Number Product Size AVAILABILITY Price Qty
FCT070
LV-PGK-Cre, 2x25uL (1x10^8 TU/mL)
2x25uL (1x10^8 CFU/mL) In stock
Regular Price:$390.00
FCT071
LV-PGK-Cre, 8x25uL (1x10^8 TU/mL)
8x25uL (1x10^8 CFU/mL) In stock
Regular Price:$980.00
Specifications

Product Type: Virus
Biosafety Level: BSL-2
Vector Information: Vector includes both 5? and 3? lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Target Gene / Reporter(s): Cre
Promoter: PGK
Selection Gene: puromycin
Virus: LV-PGK-Cre
Titer: 1 x 10^8CFU/ml
Storage: Store at -80C. Multiple freeze/thaw cycles not recommended.
Shipped: Dry ice

References

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