Recombinant Human Wild-type Alpha Thrombin
This, fully active, wild-type human alpha thrombin protein was expressed recombinantly in E. Coli and purified to >95% (by SDS-PAGE).
Thrombin is a serine protease that possesses trypsin-like behavior in that it prefers to cleave its substrates after arginine and lysine residues. It elicits functional responses critical to blood homeostasis by interacting with diverse physiological substrates.
From the laboratory of Enrico Di Cera, MD, St. Louis University.
Part of The Investigator's Annexe program.
This, fully active, wild-type human alpha thrombin protein was expressed recombinantly in E. Coli and purified to >95% (by SDS-PAGE).
Thrombin is a serine protease that possesses trypsin-like behavior in that it prefers to cleave its substrates after arginine and lysine residues. It elicits functional responses critical to blood homeostasis by interacting with diverse physiological substrates.
From the laboratory of Enrico Di Cera, MD, St. Louis University.
Part of The Investigator's Annexe program.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Protein |
| Name: | Human Alpha Thrombin (Wild-type) |
| Accession ID: | P00734, NM_000506 |
| Source: | E. coli |
| Molecular Weight: | 33.6 kDa |
| Amino Acid Sequence: | TFGSGEADCG LRPLFEKKSL EDKTERELLE SYIDGRIVEG SDAEIGMSPW QVMLFRKSPQ ELLCGASLIS DRWVLTAAHC LLYPPWDKNF TENDLLVRIG KHSRTRYERN IEKISMLEKI YIHPRYNWRE NLDRDIALMK LKKPVAFSDY IHPVCLPDRE TAASLLQAGY KGRVTGWGNL KETWTANVGK GQPSVLQVVN LPIVERPVCK DSTRIRITDN MFCAGYKPDE GKRGDACEGD SGGPFVMKSP FNNRWYQMGI VSWGEGCDRD GKYGFYTHVF RLKKWIQKVI DQFGE |
| Fusion Tag(s): | None |
| Purity: | >95% by SDS-PAGE |
| Buffer: | 300mM NaCl, 20mM Tris pH 7.4 |
| Concentration: | 0.1mg/mL |
| Activity: | Wild-type, active form. Activity is determined by using the very potent thrombin inhibitor hirudin to active site titrate the thrombin we prepared for Kerafast. Based on this titration, the material is 93% active. The uMolar active site titrated concentration is 2.91uM. If you consider the conversion of 1 NIH unit = 0.324ug of WT thrombin there are 287 units of WT thrombin in each 100ug vial. This value takes into account the 93% active site titration correction. |
| Comments: | The referenced sequences are processed to produce the alpha form of the enzyme. |
| Storage: | -80C |
| Shipped: | Dry ice |
Provider
From the laboratory of Enrico Di Cera, MD, St. Louis University.
References
- Marino F, Pelc LA, Vogt A, Gandhi PS, Di Cera E. Engineering thrombin for selective specificity toward Protein C adn PAR1. Journal of Biological Chemistry, 285(25):19145-19152, (2010).
- Dang QD, Di Cera E. A simple activity assay for thrombin and hirudin. J Protein Chem. 1994 May;13(4):367-73.
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