This N-terminal His-tagged 320-residue fragment of Histidyl-tRNA Synthetase from E. coli has been truncated immediately following motif 3.
Histidyl-tRNA synthetases attach amino acids to the 3' ends of their cognate transfer RNAs in a two-step reaction catalyzed by ATP. The accuracy of this reaction is crucial for the overall fidelity of protein synthesis. Defects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
From the laboratory of Christopher S. Francklyn, PhD, University of Vermont.
Part of The Investigator's Annexe program.
|Name:||N-Catalytic Domain Histidyl-tRNA synthetase w/6x His-tag|
|Accession ID:||Derived from AAC75567, P60906|
|Molecular Weight:||36 kDa|
|Amino Acid Sequence:||MRGSHHHHHHmakniqairgmndylpgetaiwqriegtlknvlgsygyseirlpiveqtplfkraigevtdvvekemytfedrngdsltlrpegtagcvragiehgllynqeqrlwyigpmfrherpqkgryrqfhqlgcevfglqgpdidaelimltarwwralgisehvtlelnsigslearanyrdalvafleqhkekldedckrrmytnplrvldsknpevqallndapalgdyldeesrehfaglckllesagiaytvnqrlvrgldyynrtvfewvtnslgsqgtvcaggrydglveqlggratpavgfamglerlvllvqavn|
|Fusion Tag(s):||N-terminal 6x His-tag|
|Purity:||> 95% by HisTrap|
|Buffer:||50 mM HEPES pH 7.5; 100 mM KCl; 10 mM MgCl2, 5 mM ?-mercaptoethanol, 40% glycerol|
|Storage:||Store at -20C|
Protein has limited stability in aqueous buffers without glycerol
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