Biotin-1,3-cyclopentanedione (BP1) is a non-dimedone based probe and contains a biotin tag making it compatible with several techniques and forms of analysis. At physiological pH its reactivity is comparable with dimedone and has increasing activity at lower pH.
Redox-sensitive cysteine residues in proteins may serve as important components of oxidative signaling or sensors of oxidative stress. Cysteine sulfenic acid modification is an emerging area of interest for those studying biological signal transduction within the
From the laboratory of Cristina M. Furdui, PhD, Wake Forest School of Medicine.
|Product Type:||Small Molecule|
|Format:||light yellow solid|
|Purity:||> 90% by NMR|
|Solubility:||The producing laboratory recommends using 100% DMSO to make a 100mM stock. Once dissolved, it can be aliquotted and stored at -20C to -80C for further experiments. It is recommended to avoid multiple freeze-thaw cycles. It is recommended to wait to dissolve in aqueous buffer to (typically 1 mM) until right before using in experiments.|
|Stability:||> 6 months at -20C|
|Storage:||Store at -20C. Avoid multiple freeze-thaw cycles|
Reaction products of pure protein and cell lysates with BP1. (A) ESI-TOF MS spectrum showing C165S AhpC-BP1 covalent adduct formation. (B) Western-blot analysis of proteins from NIH 3T3 cell lysates labeled with BP1. The blot was probed with streptavidin-HRP, and antibodies for AhpC and beta-actin. Samples after enrichment in proteins labeled with BP1 were probed for PLCγ1.
Adapted from: Qian, J., et al. Chem. Commun., 47, 92039205 (2011).
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