rtTA (CMV, puromycin) Lentivirus

This strain carries the tetracycline reverse transcriptional activator (rtTA) under the control of the CMV promoter. The rtTA binds to tetracycline operator elements (TetO) in the presence of doxycycline, causing transcriptional activation of genes downstream of TetO.

This premade lentivirus includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction and expression of the target genes as well as antibiotics resistant genes or reporters. A woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is believed to promote RNA processing events and nuclear export, boosting expression of your gene of interest in target cells by facilitating the production of mature mRNA from transcripts initiated by the virus's internal promoter (e.g. pCMV).

This lentivirus expressses rtTA under the control of the CMV promoter.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
FCT045
LV-CMV-rtTA (2x25 µl )
2x25uL (1x10^8 CFU/mL) In stock
Regular Price:$490.00
On Sale:
FCT047
LV-CMV-rtTA (8x25µl)
8x25uL (1x10^8 CFU/mL) In stock
Regular Price:$1,200.00
On Sale:
Specifications

Product Type: Virus
Vector Information: Vector includes both 5? and 3? lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Target Gene / Reporter(s): rtTA
Promoter: CMV
Selection Gene: Puromycin
Virus: rtTA
Titer: 1 x 10^7-8 CFU/ml
Storage: Store at -80C. Multiple freeze/thaw cycles not recommended.
Shipped: Dry ice

References

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