GFP (CMV) Lentivirus

Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

This premade lentivirus includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction and expression of the target genes as well as antibiotics resistant genes or reporters. A woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is believed to promote RNA processing events and nuclear export, boosting expression of your gene of interest in target cells by facilitating the production of mature mRNA from transcripts initiated by the virus's internal promoter (e.g. pCMV).

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
FCT003
LV-CMV-GFP (2x25 µl )
2x25uL (1x10^8 CFU/mL) In stock
Regular Price:$390.00
On Sale:
FCT004
LV-CMV-GFP ( 8x25 µl )
8x25uL (1x10^8 CFU/mL) In stock
Regular Price:$980.00
On Sale:
Specifications

Product Type: Virus
Biosafety Level: BSL-2
Vector Information: Vector includes both 5? and 3? lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Target Gene / Reporter(s): GFP
Promoter: CMV
Selection Gene: puromycin
Virus: LV-CMV-GFP
Titer: 1 x 10^8CFU/ml
Storage: Store at -80C. Multiple freeze/thaw cycles not recommended.
Shipped: Dry ice

References
  1. Xu R, Paul BD, Smith DR, Tyagi R, Rao F, Khan AB, Blech DJ, Vandiver MS, Harraz MM, Guha P, Ahmed I, Sen N, Gallagher M, Snyder SH. Inositol polyphosphate multikinase is a transcriptional coactivator required for immediate early gene induction. Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16181-6. View Article
  2. Kim DH, Lee D, Chang EH, Kim JH, Hwang JW, Kim JY, Kyung JW, Kim SH, Oh JS, Shim SM, Na DL, Oh W, Chang JW. GDF-15 secreted from human umbilical cord blood mesenchymal stem cells delivered through the cerebrospinal fluid promotes hippocampal neurogenesis and synaptic activity in an Alzheimer's disease model. Stem Cells Dev. 2015 Oct 15;24(20):2378-90. View Article
  3. Himburg HA, Doan PL, Quarmyne M, Yan X, Sasine J, Zhao L, Hancock GV, Kan J, Pohl KA, Tran E, Chao NJ, Harris JR, Chute JP. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med. 2017 Jan;23(1):91-99. View Article
  4. Blair JD, Hockemeyer D, Bateup HS. Genetically engineered human cortical spheroid models of tuberous sclerosis. Nat Med. 2018 Oct;24(10):1568-1578. View Article

If you publish research with this product, please let us know so we can cite your paper.

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