Glucokinase (GK; EC 220.127.116.11) is an enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Glucokinase occurs in cells in the liver, pancreas, gut, and brain of humans and most other vertebrates. In each of these organs it plays an important role in the regulation of carbohydrate metabolism by acting as a glucose sensor, triggering shifts in metabolism or cell function in response to rising or falling levels of glucose, such as occur after a meal or when fasting. Mutations of the gene for this enzyme can cause unusual forms of diabetes or hypoglycemia.
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|Molecular Weight:||52.8 kDa|
|Buffer:||50 mM potassium phosphate pH-7.4, 50 mM sodium chloride, 0.5 mM ethylenediaminetetraaceticacid, and 2.5% glycerol.|
|Comments:||Human pancreatic glucokinase, (Accession No. NP_000153), aa 470 (full length) with C-terminal FLAG Tag, MW= 52.80 kDa, expressed in E.coli.|
|Storage:||Stable >6 months -80C|
Specific Activity: 303 pmol/min/ug. One unit is defined as the amount of enzyme that will convert 1 nmol of NADP to NADPH at 30 oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30 oC for 30 min.
Activation: (±) Ro 28-0450 (100 uM) activated human pancreatic glucokinase 865% verses control measured at glucose [S]0.5.
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