DCP-Bio1 is dimedone based and contains a biotin tag making it compatible with several techniques and forms of analysis.
Redox-sensitive cysteine residues in proteins may serve as important components of oxidative signaling or sensors of oxidative stress. Cysteine sulfenic acid modification is an emerging area of interest for those studying biological signal transduction within the cell.
Cysteine sulfenic acid formation in proteins results from the oxidative modification of susceptible cysteine residues by mild oxidizing agents such as hydrogen peroxide, alkyl hydroperoxides, and peroxynitrite. These sulfenic acid modified proteins can be identified by their ability to form adducts with dimedone, but this reagent provides no spectral or affinity tag to such adduct to allow for later analysis. DCP-Bio1 can be used to effectively detect the formation of cysteine sulfenic acid in the redox regulation of proteins, and with the presence of a biotin label, DCP-Bio1 is compatible with several techniques and forms of analysis post-labeling.
|Product Type:||Small Molecule|
|Name:||DCP-Bio1; 3-(2,4-dioxocyclohexyl)propyl 5-((3aR,6S,6aS)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-6-yl)pentanoate|
|Molecular Weight:||396.5 g/mol|
|Purity:||>98% pure, see Poole, et al., 2007|
|Solubility:||At least 500 mM in DMSO, at least 5 mg/ml in acetonitrile|
|Stability:||stable > 6 months at -20 degC|
|Spectral Information:||No visible absorbance; NMR data, etc. in Poole et al., 2007|
|Storage:||room temperature for short term, -20 degC for long term|
Stock solution in DMSO can be added to cell lysis buffer, preferrably keeping final [DMSO] < 2% for labeling proteins. Can be dissolved in acetonitrile to prepare aliquots and redry.
DCP-Bio1 Application References
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