GFP-Sunbody Plasmids
GFP-Sunbody Plasmid for expression of GFP-tagged light switchable nanobody against mCherry (Light-ON switch).
Highlights:
- Responds to photons (within seconds) emitting in the range of 400-500nm
- Can be used to precisely control protein degradation, gene transcription, and base editing by harnessing the power of light (as demonstrated in He, Lian, et al. Advanced Biology (2021): 2000541)
- 2xGFP-Sunbody Plasmid also available, a concatemer of Sunbody, with enhanced strength of light-switchable antibody-antigen binding against mCherry
Intracellular single-domain antibodies (intrabodies) and their mimetics derived from synthetic protein scaffolds (e.g., nanobodies and monobodies) are used with the aim to overcome shortcomings of natural antibody molecules. Due to their smaller size, no disulfide-bond formation and ease of in vitro and cellular expression, nanobodies may serve as useful tools for both research and potential diagnostic/therapeutic applications. Several classes of chemically or light-dependent single-domain antibodies have been produced through recent engineering efforts.
From the laboratory of Yubin Zhou, MD, PhD, Texas A&M University.
GFP-Sunbody Plasmid for expression of GFP-tagged light switchable nanobody against mCherry (Light-ON switch).
Highlights:
- Responds to photons (within seconds) emitting in the range of 400-500nm
- Can be used to precisely control protein degradation, gene transcription, and base editing by harnessing the power of light (as demonstrated in He, Lian, et al. Advanced Biology (2021): 2000541)
- 2xGFP-Sunbody Plasmid also available, a concatemer of Sunbody, with enhanced strength of light-switchable antibody-antigen binding against mCherry
Intracellular single-domain antibodies (intrabodies) and their mimetics derived from synthetic protein scaffolds (e.g., nanobodies and monobodies) are used with the aim to overcome shortcomings of natural antibody molecules. Due to their smaller size, no disulfide-bond formation and ease of in vitro and cellular expression, nanobodies may serve as useful tools for both research and potential diagnostic/therapeutic applications. Several classes of chemically or light-dependent single-domain antibodies have been produced through recent engineering efforts.
From the laboratory of Yubin Zhou, MD, PhD, Texas A&M University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Plasmid |
| Gene/insert name: | GFP-Sunbody, 2x (GFP-Sunbody) |
| Accession ID: | P42212 |
| Organism: | Synthetic |
| Antibiotic Resistance: | Amp |
| Fusion Tag(s): | GFP |
| Grow in E. coli at 37 C: | Yes |
| Cloning Site 5': | NcoI |
| Cloning Site 3': | XhoI |
| Insert Size: | 1953bp (GFP-Sunbody); 3918bp (2xGFP-Sunbody) |
| Vector Backbone and Size: | pTriEx, 4938bp |
| High or low copy: | Low |
| Storage: | -20C |
| Shipped: | Room temperature |
- He L, Tan P, Huang Y, Zhou Y. Design of Smart Antibody Mimetics with Photosensitive Switches. Adv Biol (Weinh). 2021 May;5(5):e2000541. doi: 10.1002/adbi.202000541. Epub 2021 Feb 5. PMID: 34028213; PMCID: PMC8295464.
If you publish research with this product, please let us know so we can cite your paper.
