Hepatitis Delta Virus (HDV), Pan-Genotypic Antibodies

This mouse monoclonal Anti-Hepatitis Delta Virus (HDV) antibody was raised against Hepatitis Delta Antigen (HDAg). The two antibody clones, FD3A7 & FD5C4, were screened to ensure pan-genotypic specificity.

Highlights:

  • Specifically detect HDAg of all 8 viral genotypes
  • Validated in ELISA, western blot, immunofluorescence and in a competitive diagnostic ELISA using serum of HDV-infected patients

Chronic Hepatitis Delta Virus (HDV) infections represent the most severe form of viral hepatitis with about 20 million infected individuals worldwide. HDV is a satellite virus of Hepatitis B Virus (HBV), which means that all patients chronically infected with HDV are always co-infected with HBV. There are 8 different HDV genotypes described worldwide. The HDV genome encodes for only one protein: the Hepatitis Delta Antigen (HDAg).

From the laboratory of Stephan Urban, PhD , Heidelberg University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EHD001
Anti-Hepatitis Delta Virus (HDV), Pan-Genotypic [FD3A7] Antibody
5mL (supernatant) In stock
Regular Price:$269.00
On Sale:
EHD002
Anti-Hepatitis Delta Virus (HDV), Pan-Genotypic [FD5C4] Antibody
5mL (supernatant) In stock
Regular Price:$269.00
On Sale:
Specifications

Product Type: Antibody
Name: Anti-Hepatitis Delta Virus (HDV), Pan-Genotypic [FD3A7] Antibody
Accession ID: Synthetic, chimeric sequence (consensus of all 8 genotypes). Most similarity (93%) with Genbank SCC98289
Antigen: Hepatitis Delta Antigen (HDAg)
Molecular Weight: 25kD
Isotype: IgG1 kappa (FD3A7) and IgG2b kappa (FD5C4)
Clonality: Monoclonal
Clone Name: FD3A7 and FD5C4
Reactivity: Virus (Hepatitis Delta Virus)
Immunogen: Full-length protein
Species Immunized: Mouse, BALB/c
Buffer: Cell culture supernatant
Tested Applications: WB, IF 1:100 (may be optimized further if needed)
Storage: -80C (Avoid multiple freeze/thaw cycles)
Shipped: Dry ice

Data
data

(A) Western blot. Lysates of cells expressing HDAg of different genotypes was separated by SDSPAGE, blotted and incubated with the mAb FD3A7 at 1:500 dilution. (B) Immunofluorescence. HuH7 cells were transfected with plasmids encoding HDAg of different HDV genotypes. Cells were fixed, permeabilized and incubated with the two mAb clones at 1:20-500 dilution. A goatanti-mouse-Alexa545 antibody was used for detection. Both mAb clones detect HDAg of all HDV genotypes.

Provider
From the laboratory of Stephan Urban, PhD, Heidelberg University.
References
  1. Wang W, Lempp FA, Schlund F, Walter L, Decker CC, Zhang Z, Ni Y, Urban S. Assembly and infection efficacy of hepatitis B virus surface protein exchanges in 8 hepatitis D virus genotype isolates. J Hepatol. 2021 Aug;75(2):311-323. doi: 10.1016/j.jhep.2021.03.025. Epub 2021 May 5. PMID: 33845061.

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