This mouse IgG monoclonal antibody was generated against purified his-tagged BpaB and is specific for Burkholderia mallei autotransporter BpaB.
Recombinant versions available from our sister company, Absolute Antibody:
Autotransporter proteins (AT) form one of the largest class of virulence factors in Gram-negative organisms and perform important functions in pathogenesis including flocculation, formation of biofilms , complement resistance, host cell adhesion and entry, intracellular motility and replication, cytotoxicity, and lipolytic activity. Given their function in pathogenesis and overall structure, AT are excellent targets for developing medical countermeasures (MCM) against pathogenic organisms. A significant portion of AT (passenger domain) is readily accessible for recognition by the immune system as it is exposed on the bacterial surface.
|Name:||Burkholderia mallei BpaB [BpaB#4] Antibody|
|Antigen:||BpaB autotransporter of B. mallei|
|Molecular Weight:||105 kDa per subunit|
Recombinant versions: see product name
|Immunogen:||Purified, his-tagged BpaB|
EGA074: Cell culture supernatant
Recombinant versions: PBS with 0.02% Proclin 300
|Tested Applications:||ELISA, Immunofluorescence, Western Blot and Flow Cytometry|
BpaB production by E. coli recombinant strains. Equivalent amounts of whole cell lysates (WCL), total membrane proteins (TMP) and sarkosyl-insoluble fractions containing OM proteins (OMP) were resolved by SDS-PAGE, transferred to PVDF membranes and analyzed by western blot with the monoclonal antibody BpaB-MAb#4. Molecular weight markers are shown to the left in kilodaltons.
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