Anti-dsRNA [rJ2] Antibody

This mouse IgG2a monoclonal antibody [rJ2], recognizes double stranded RNA (dsRNA) of greater than 40 bp in length which is generated during the replication of positive sense genome viruses.


  • Reacts with dsRNA of positive sense genome viruses
  • Suitable for Immunoflourescence, Immunohistochemistry, Immuno-EM and Affinity Purification applications

Recombinant versions available from our sister company, Absolute Antibody:

rJ2 antibody can be used to detect dsRNA intermediates of viruses as diverse as Zika virus, Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.

For more information on how this antibody reacts with Zika virus see a blog post from ASM: Zika Virus Inside Cells

From the laboratory of Abraham L. Brass, MD, PhD, University of Massachusetts Medical School.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Anti-dsRNA [rJ2] Antibody
Supernatant, 4mL (2x2mL) Unavailable
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Recombinant Anti-dsRNA [J2], Mouse, Fab fragment
100ug In stock
Regular Price:$630.00
On Sale:
Recombinant Anti-dsRNA [J2], Mouse, IgG2ak
200ug In stock
Regular Price:$470.00
On Sale:
Recombinant Anti-dsRNA [J2], Rabbit, IgGk
200ug In stock
Regular Price:$470.00
On Sale:

Product Type: Antibody
Name: Anti-dsRNA [rJ2] Antibody
Antigen: double stranded RNA produced by positive sense genome viruses
Molecular Weight: dsRNA > 40 bp in length
Isotype: ES2001: IgG2a kappa
Recombinant versions: see product name
Clonality: Monoclonal
Fusion Tag(s): His-Tagged
Clone Name: Recombinant J2 (rJ2)
Reactivity: Positive sense genome viruses
Specificity: Positive sense genome viruses
Immunogen: dsRNA
Species Immunized: Mouse
Epitope: dsRNA from positive sense genome virus replication
Buffer: ES2001: Culture medium, DMEM and 5% FBS
Recombinant versions: PBS with 0.02% Proclin 300
Tested Applications: IF (1:60), IHC, immuno-EM, Affinity purification
Storage: ES2001: -80C
Recombinant versions: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C
Shipped: ES2001: Dry ice
Recombinant versions: Cold packs


Zika Virus Infecting Cells

Anti-dsRNA [rJ2] Antibody used at 1:60 to detect Zika virus (green) infection of cultured human cells. Zika virus levels are lower (top right panel), as UMMS researchers decreased the levels of the EMC1 protein in the human cells using siRNA. NT= non-targeting negative control siRNA. Cell nuclei are shown in blue below

Adapted from: Savidis G, et al. Cell Reports June 21 2016

From the laboratory of Abraham L. Brass, MD, PhD, University of Massachusetts Medical School.
  1. Savidis G, Perreira JM, Portmann JM, Meraner P, Guo Z, Green S, Brass AL. The IFITMs Inhibit Zika Virus Replication. Cell Rep. 2016 Jun 14;15(11):2323-30. View Article
  2. George Savidis, William M. Mcdougall, Paul Meraner, Jill M. Perreira, Jocelyn M. Portmann, Gaia Trincucci, Sinu P. John, Aaron M. Aker, Nicholas Renzette, Douglas R. Robbins, Zhiru Guo, Sharone Green, Timothy F. Kowalik, Abraham L. Brass. Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. Cell Reports, June 2016 DOI: 10.1016/j.celrep.2016.06.028
  3. Samuelsen S, Jørgensen CD, Mellins ED, Torok KS, Astakhova K. Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens. PLoS One. 2018 Apr 11;13(4):e0195381. View Article
  4. Shelkovnikova TA, Kukharsky MS, An H, Dimasi P, Alexeeva S, Shabir O, Heath PR, Buchman VL. Protective paraspeckle hyper-assembly downstream of TDP-43 loss of function in amyotrophic lateral sclerosis. Mol Neurodegener. 2018 Jun 1;13(1):30. View Article
  5. Jena KK, Mehto S, Nath P, Chauhan NR, Sahu R, Dhar K, Das SK, Kolapalli SP, Murmu KC, Jain A, Krishna S, Sahoo BS, Chattopadhyay S, Rusten TE, Prasad P, Chauhan S, Chauhan S. Autoimmunity gene IRGM suppresses cGAS-STING and RIG-I-MAVS signaling to control interferon response. EMBO Rep. 2020 Sep 3;21(9):e50051.  View article
  6. Yin X, Riva L, Pu Y, Martin-Sancho L, Kanamune J, Yamamoto Y, Sakai K, Gotoh S, Miorin L, De Jesus PD, Yang CC, Herbert KM, Yoh S, Hultquist JF, García-Sastre A, Chanda SK. MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells. Cell Rep. 2021 Jan 12;34(2):108628. View article 

If you publish research with this product, please let us know so we can cite your paper.