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Specifications
| Product Type: | Protein |
| Source: | E. coli |
| Molecular Weight: | 12.24 kDa |
| Purity: | > 95% (densitometry) |
| Buffer: | 50 mM HEPES-pH 7.5, 0.5 mM DTT, 0.5 mM EDTA, and 2.5 % glycerol. |
| Concentration: | 15.4mg/mL |
| Storage: | Stable > 12 months -80C |
| Shipped: | Dry ice |
Comments
SDS-Page:
| Binding Characteristics: Assay conditions: 50 mM HEPES, pH 7.8, +50 nM FKBP12, plus titrated FK506 (titration range 200-3.125 nM) incubated at 24C for 30 min. Add 200 ?M substrate (suc-Ala-Phe-Pro-Phe-pNA) followed by addition of chymotrypsin (50 ?g/ml) in a final volume of 100 ?l. Absorbance (A390) was then measured every 4 sec at 24oC for 2 min. Inhibition values were calculated by comparing the rates of FKBP12-specific substrate cleavage in the presence and absence of FK506. FK506 IC50 = 37 nM. |  |
| Crystallography: apo FKBP12 crystals (right) diffract to 1.6 Å resolution using in-house X-ray source. |  |
References
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