Mouse Fibroblast, COX10 Knock-Out Cell Line

Mouse fibroblast cell line lacking the gene cytochrome c oxidase assembly factor heme (COX10).

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. To generate the cell line, a primary skin fibroblast culture was stablished from a knockin mouse homozygous for the floxed COX10 (exon 6). Cells were immortalized by retroviral infection of E6-E7 type 16 papiloma virus carrying G418 resistance. To generate COX10 KO, immortalized cells were transfected with plasmid expressing cre recombinase and clones were obtained after hygromycine selection. This is KO clone KO#19. Generation and characterization of cell line is described in PMID: 16782876. Mouse used to generate cell line had mixed genetic background (129svj and C57BL6J).

From the laboratory of Carlos T. Moraes, PhD, University of Miami.

Catalog Number Product Size AVAILABILITY Price Qty
EMI019
Mouse Fibroblast, COX10 Knock-Out Cell Line
1 vial 3-5 weeks
Price: $599.00
Specifications
Product Type: Cell Line
Cell Type: Skin Fibroblast
Organism: Mouse
Source: Skin
Morphology: Fibroblast
Biosafety Level: BSL 1
Subculturing: Split when confluent
Growth Conditions: High Glucose Dulbecco's modified Eagle's medium containing 10% fetal bovine serum, 1 mM pyruvate, 50 ug/ml uridine and gentamicin
Cryopreservation: Growing media with 60% FBS and 10% DMSO
Comments: E6/E7 immortalized fibroblasts
Storage: LN2
Shipped: Dry Ice
Provider
From the laboratory of Carlos T. Moraes, PhD, Universityof Miami.
References
  1. Diaz F, Fukui H, Garcia S, Moraes CT. Cytochrome c oxidase is required for the assembly/stability of respiratory complex I in mouse fibroblasts. Mol Cell Biol. 2006 Jul;26(13):4872-81. PubMed PMID: 16782876; PubMed Central PMCID: PMC1489173.

If you publish research with this product, please let us know so we can cite your paper.

 
Loading...
Loading...