Protein-Nucleic Acid Complex Crystal Screen

Catalog Number	Product	Size	AVAILABILITY	Price	Qty
EE0001	Protein-Nucleic Acid Complex Crystal Screen	48 conditions, 1.5 mL/condition	3-5 weeks	Price: $215.00

Specifications

Product Type:	Kit
Storage:	4C (short term), -20C (long term)
Shipped:	Ambient temperature

Structures solved using Protein-Nucleic Acid Complex Crystal Screen

	Transcription factor AmrZ in complex with dsDNA PDB ID: 3QOQ Pryor EE Jr, Waligora EA, Xu B, Dellos-Nolan S, Wozniak DJ, et al. The Transcription Factor AmrZ Utilizes Multiple DNA Binding Modes to Recognize Activator and Repressor Sequences of Pseudomonas aeruginosa Virulence Genes. PLoS Pathogens. 2012; 8(4): e1002648.
	DNA methyl lesion repair enzyme AlkB in complex with ssDNA PDB ID: 3KHC Holland PJ, Hollis T. Structural and mutational analysis of Escherichia coli AlkB provides insight into substrate specificity and DNA damage searching. PLoS ONE. 2010; 5(1):e8680.
	3' to 5' exonuclease TREX1 in complex with ssDNA PDB ID: 2OA8 de Silva U, Choudhury S, Bailey SL, Harvey S, Perrino FW, Hollis T. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem. 2007; 282(14):10537-43.

References

Pryor EE Jr, Wozniak DJ, Hollis, T. Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein-DNA crystals. Acta Crystallographica Section F. 2012; F68: 985-993.
Pryor EE Jr, Waligora EA, Xu B, Dellos-Nolan S, Wozniak DJ, et al. The Transcription Factor AmrZ Utilizes Multiple DNA Binding Modes to Recognize Activator and Repressor Sequences of Pseudomonas aeruginosa Virulence Genes. PLoS Pathogens. 2012; 8(4): e1002648.
Holland PJ, Hollis T. Structural and mutational analysis of Escherichia coli AlkB provides insight into substrate specificity and DNA damage searching. PLoS ONE. 2010; 5(1): e8680.
de Silva U, Perrino FW, Hollis T. DNA binding induces active site conformational change in the human TREX2 3'-exonuclease. Nucleic Acids Res. 2009; 37(7):2411-2417.
de Silva U, Choudhury S, Bailey SL, Harvey S, Perrino FW, Hollis T. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem. 2007; 282(14):10537-43
Hollis T. Crystallization of protein-DNA complexes. In Methods Mol Biol. (ed. Doublie) 2007; 363():225-237.

Data

Structures solved using Protein-Nucleic Acid Complex Crystal Screen

	Transcription factor AmrZ in complex with dsDNA PDB ID: 3QOQ Pryor EE Jr, Waligora EA, Xu B, Dellos-Nolan S, Wozniak DJ, et al. The Transcription Factor AmrZ Utilizes Multiple DNA Binding Modes to Recognize Activator and Repressor Sequences of Pseudomonas aeruginosa Virulence Genes. PLoS Pathogens. 2012; 8(4): e1002648.
	DNA methyl lesion repair enzyme AlkB in complex with ssDNA PDB ID: 3KHC Holland PJ, Hollis T. Structural and mutational analysis of Escherichia coli AlkB provides insight into substrate specificity and DNA damage searching. PLoS ONE. 2010; 5(1):e8680.
	3' to 5' exonuclease TREX1 in complex with ssDNA PDB ID: 2OA8 de Silva U, Choudhury S, Bailey SL, Harvey S, Perrino FW, Hollis T. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem. 2007; 282(14):10537-43.

Provider

From the laboratory of Thomas Hollis, PhD, Wake Forest School of Medicine.

References

Pryor EE Jr, Wozniak DJ, Hollis, T. Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein-DNA crystals. Acta Crystallographica Section F. 2012; F68: 985-993.
Pryor EE Jr, Waligora EA, Xu B, Dellos-Nolan S, Wozniak DJ, et al. The Transcription Factor AmrZ Utilizes Multiple DNA Binding Modes to Recognize Activator and Repressor Sequences of Pseudomonas aeruginosa Virulence Genes. PLoS Pathogens. 2012; 8(4): e1002648.
Holland PJ, Hollis T. Structural and mutational analysis of Escherichia coli AlkB provides insight into substrate specificity and DNA damage searching. PLoS ONE. 2010; 5(1): e8680.
de Silva U, Perrino FW, Hollis T. DNA binding induces active site conformational change in the human TREX2 3'-exonuclease. Nucleic Acids Res. 2009; 37(7):2411-2417.
de Silva U, Choudhury S, Bailey SL, Harvey S, Perrino FW, Hollis T. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem. 2007; 282(14):10537-43
Hollis T. Crystallization of protein-DNA complexes. In Methods Mol Biol. (ed. Doublie) 2007; 363():225-237.
Sugiman-Marangos S, Junop M. Crystallization of the DdrB-DNA complex from Deinococcus radiodurans. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1534-7. doi: 10.1107/S1744309112044041. PubMed PMID: 23192041; PubMed Central PMCID: PMC3509982. View Article
Choy WW, Datta D, Geiger CA, Birrane G, Grant MA. Crystallization and preliminary X-ray analysis of a complex of the FOXO1 and Ets1 DNA-binding domains and DNA. Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):44-8. View Article
Hadzi S, Garcia-Pino A, Gerdes K, Lah J, Loris R. Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module. Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):226-33. View Article
Malaby AW, Martin SK, Wood RD, Doublié S. Expression and Structural Analyses of Human DNA Polymerase θ (POLQ). Methods Enzymol. 2017;592:103-121. View Article

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References

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