Anti-CD20, N-Terminal [1439] Antibody

This rabbit polyclonal antibody [1439] was generated against a synthetic peptide corresponding to residues 25-41 (SGPKPLFRRMSSLVGPT) of human CD20 conjugated to ovalbumin, and is specific for the intracellular N-terminal region of human CD20.

Highlights:

  • Reacts with intracellular N-terminal residues 25-41 of human CD20
  • Valuable for both pre-clinical and clinical research applications
  • Suitable for Western Blot applications

CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies.

From the laboratory of Julie P. Deans, PhD, University of Calgary.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EUC006
Anti-CD20, N-Terminal [1439] Antibody, 100uL
100uL In stock
Price: $299.00
Specifications
Product Type: Antibody
Antigen: CD20 (CD20-N)
Molecular Weight: 33-35 kDa
Clonality: Polyclonal
Clone Name: 1439
Reactivity: Human
Immunogen: Peptide conjugated to ovalbumin
Species Immunized: Rabbit
Epitope: N-terminal residues 25-41; SGPKPLFRRMSSLVGPT
Buffer: Serum
Tested Applications: WB (1:10,000), IP
Storage: -80C
Shipped: Dry ice
Data

Western Blot of CD20 N and C-terminus

Western blots of human Ramos B-cells lysates probed with rabbit anti-CD20N (targeting the N-terminal residues 25-41) and rabbit anti-CD20C (targeting the C-terminal residues 231-245), pre-incubated with immunizing peptide (lanes 2 and 5), irrelevant peptide (lanes 3 and 6) or no peptide (lanes 1 and 4).

Adapted from: Polyak MJ, et al. J Immunol. 1998 Oct 1;161(7):3242-8.

Provider
From the laboratory of Julie P. Deans, PhD, University of Calgary.
References
  1. Polyak MJ, Tailor SH, Deans JP. Identification of a cytoplasmic region of CD20 required for its redistribution to a detergent-insoluble membrane compartment. J Immunol. 1998 Oct 1;161(7):3242-8.

If you publish research with this product, please let us know so we can cite your paper.

 
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