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Specifications
| Product Type: | Protein |
| Name: | Isocitrate Dehydrogenase 1 (IDH1)-(R132C variant)-TEV-8xHis |
| Source: | Human protein expressed in E.Coli Rosetta (DE3) |
| Molecular Weight: | 48,669 Da |
| Amino Acid Sequence: | MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIG(R>C)CHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL^ENLYFQGGHHHHHHHHG^ denotes begining of TEV cleavage site |
| Fusion Tag(s): | Contains C-terminal 8xHis tag with TEV cleavage site |
| Purity: | >98% as determined by SDS-PAGE |
| Buffer: | 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM BME, 10% glycerol |
| Storage: | -80C |
| Shipped: | Dry ice |
Documentation
Additional Information
Theoretical pI: 6.50
Extinction coefficient: 65320
A280: 1.342
Data
SDS-PAGE Analysis
References
- Dang, L. et al. Cancer associated IDH1 mutations produce 2-hydroxyglutarate. Nature 462, 739-744 (2009).
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