Anti-LRP6 [1C10] Antibody

This IgG1 mouse mononoclonal antibody was generated against the cytoplasmic domain of human LRP6 and is reactive against human, mouse, and rat LRP6.

Low density lipoprotein receptor-related protein 6 (LRP6) is a member of the low density lipoprotein receptor gene family (LDLR).  LRP6 a cell surface protein, plays a critical role in embryonic development by functioning as a co-receptor specifically for the ß-catenin Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. When LRP6 binds to Wnt, it phosphorylates Wnt at multiple sites, which results in the recruitment of the scaffolding protein Axin to the cell membrane and ß catenin activation. LRP6 is the receptor for a novel Wnt antaganoist, DKK1. LRP6 is associated with autosomal dominant coronary artery disease type 2 and bone formation, as well as many human malignancies.

From the laboratory of Stuart A. Aaronson, MD, Icahn School of Medicine at Mount Sinai.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EMS201
Anti-LRP6 [1C10] Antibody, 100ug
100ug In stock
Price: $278.00
Specifications
Product Type: Antibody
Name: Anti-LRP6 [1C10] Antibody
Accession ID: AAI43726.1, O75581
Molecular Weight: 179 kDa
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 1C10
Reactivity: Human, Mouse, Rat
Immunogen: Cytoplasmic Domain of Human LRP6
Species Immunized: Mouse
Purification Method: Protein G Affinity Chromatography
Buffer: PBS, pH 7.4
Tested Applications: WB (1:500), IP (2ug)
Concentration: 1 mg/mL
Storage: -20C
Shipped: Cold packs
Data

Western Blot Analysis

WB Data

Western blot (100µg) and immuno-precipitation (2mg) analysis of total cell lysates from 293T cells utilizing the LRP6 mAb.

Provider
From the laboratory of Stuart A. Aaronson, MD, Icahn School of Medicine at Mount Sinai.
References
  1. Khan Z, Vijayakumar S, de la Torre TV, Rotolo S, Bafico A. Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor. Mol Cell Biol 27:7291-301 (2007).
  2. Goel S1, Chin EN, Fakhraldeen SA, Berry SM, Beebe DJ, Alexander CM.Both LRP5 and LRP6 Receptors Are Required to Respond to Physiological Wnt Ligands in Mammary Epithelial Cells and Fibroblasts. J Biol Chem 287:16454-66 (2012).
  3. van Dinther M, Zhang J, Weidauer SE, Boschert V, Muth EM, Knappik A, de Gorter DJ, van Kasteren PB, Frisch C, Mueller TD, ten Dijke P. Anti-Sclerostin antibody inhibits internalization of Sclerostin and Sclerostin-mediated antagonism of Wnt/LRP6 signaling. PLoS One 8:e62295 (2013).

If you publish research with this product, please let us know so we can cite your paper.

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