Prader-Willi Syndrome [PWS1-7] Human Induced Pluripotent Stem Cells

These induced pluripotent stem cells (iPSCs) have been derived from previously banked fibroblasts from a female individual with Prader-Willi syndrome (PWS). Like the person they were derived from, these iPSCs have an approximately 5 Mb deletion of paternal 15q11.2-q13.1.

Prader-Willi (PWS) is a neurogenetic disorder in which individuals first present with severe hypotonia and infantile failure to thrive, but later develop severe hyperphagia that leads to morbid obesity. They also have small stature, hands, and feet, behavioral problems, and persistent hypotonia. PWS is most often caused by deletion of the paternally-inherited allele of 15q11-q13.

From the laboratories of Stormy J. Chamberlain, PhD and Marc Lalande, PhD, University of Connecticut.

Catalog Number Product Size AVAILABILITY Price Qty
Prader-Willi Syndrome [PWS1-7] Human iPSCs (Feeder layer-dependent)
Live cells in T25 flask 3-5 weeks
Price: $375.00
Prader-Willi Syndrome [PWS1-7] Human iPSCs (Feeder layer-independent)
Live cells in T25 flask 4-6 weeks
Price: $575.00

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Product Type: Cell Line
Name: PWS1-7
Cell Type: Induced pluripotent stem cell (iPSC)
Accession ID: CVCL_U529
Organism: Human
Source: Fibroblasts
Morphology: Small round cells that grow in colonies, indistinguishable from human embryonic stem cells
Biosafety Level: 2
Subculturing: Manually split no more than 1:5, depending on density of dish
Growth Conditions: Feeder Layer-Dependent: (irradiated MEF (Gibco A34181 or equivalent), hESC medium: DMEM/F12 (Gibco 11330- 057 or equivalent) with 20% Knockout Serum Replacement (Invitrogen 10828-028), 1X Non-essential amino acids, 2mM L-glutamine, 7 microLiters/mL 2-Mercaptoethanol, 4ug/mL basic Fibroblast Growth Factor)
Feeder Layer-Independent: Matrigel (Corning 356231) and mTeSR (Stem Cell Technologies 05850)
Cryopreservation: 60% DMEM/F12, 30% FBS, 10% DMSO
Storage: Cells should be cultured at 37C upon arrival. Cryopreserved cells should be stored in Liquid nitrogen
Shipped: Ambient temperature as live cells in T25 flask
From the laboratories of Stormy J. Chamberlain, PhD and Marc Lalande, PhD, University of Connecticut.

These cells have been propagated using irradiated mouse embryonic fibroblasts as a feeder layer. It is recommended to keep the cells on a feeder layer until established.

  1. Chamberlain SJ, Chen PF, Ng KY, Bourgois-Rocha F, Lemtiri-Chlieh F, Levine ES, Lalande M. Induced pluripotent stem cell models of the genomic imprinting disorders Angelman and Prader-Willi syndromes. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17668-73.

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