Human Monocyte Derived Dendritic Cells
These monocyte derived dendritic cells can be utilized in a variety of applications, ranging from vaccinology to MLR and ADCC assays.
Dendritic cells play a major role in recognizing threats in vivo and directing the immune system's response. Because dendritic cells in blood are small in number, they are typically obtained by the differentiation of peripheral blood derived monocytes in the presence of IL4 and GM-CSF.
These monocyte derived dendritic cells can be utilized in a variety of applications, ranging from vaccinology to MLR and ADCC assays.
Dendritic cells play a major role in recognizing threats in vivo and directing the immune system's response. Because dendritic cells in blood are small in number, they are typically obtained by the differentiation of peripheral blood derived monocytes in the presence of IL4 and GM-CSF.
Specifications
| Product Type: | Cell Line |
| Name: | Immature dendritic cells |
| Cell Type: | Monocyte derived dendritic cells |
| Source: | Human blood monocytes |
| Organism: | Human |
| Biosafety Level: | 2 |
| Growth Conditions: | Cells can survive up to 7 days in medium containing IL4 & GM-CSF as immature dendritic cells (recommend Cellgenix DC medium with 350 units/mL IL4 and GM-CSF, cells seeded at 400-600 cells/cm2); can be further differentiated into mature dendritic cells |
| Cryopreservation: | Cryostor CS10 |
| Comments: | Dendritic cells are negative for Hepatitis B, Hepatitis C, and HIV. Cells should still be treated as potentially infectious. |
| Storage: | LN |
| Shipped: | Dry Ice |
References
- Bender A, Sapp M, Schuler G, Steinman RM, Bhardwaj N. Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J Immunol Methods. 1996 Sep 27;196(2):121-35. PubMed PMID: 8841451.
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