Hepatitis A Virus (HAV) Strain HM175 Expressing Plasmid

The plasmid pHM175/18f.2 encodes for infectious molecular clone of cell culture-adapted, rapidly replicating, cytopathic human hepatitis A virus, genotype 1a, strain HM175/18f.

Hepatitis A is an infectious disease of the liver caused by the hepatitis A virus (HAV). It is usually spread by eating food or drinking water contaminated with infected feces. Symptoms are similar to influenza and include nausea, vomiting, diarrhea, jaundice, fever, and abdominal pain. Hepatitis A vaccine is routinely available and effective.

From the laboratory of Stanley M. Lemon, MD, University of North Carolina at Chapel Hill.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Hepatitis A Virus (HAV) Strain HM175 Expressing Plasmid
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Product Type: Plasmid
Gene/insert name: Hepatitis A virus genotype 1a, variant 18f
Accession ID: GenBank: KP879216.1
Antibiotic Resistance: Ampicillin
Fusion Tag(s): None
Grow in E. coli at 37 C: Yes
Selectable markers: None
Cloning Site 5': HindIII (destroyed)
Cloning Site 3': SphI (destroyed)
Insert Size: 7531, including 3? poly(A) tract
Vector Backbone and Size: pGEM3 (modified) (2844)
High or low copy: High
Comments: Linearize plasmid DNA with MluI for T7 transcript production.
Storage: -20C
Shipped: Room Temperature

From the laboratory of Stanley M. Lemon, MD, University of North Carolina at Chapel Hill.

  1. Lemon SM, Murphy PC, Shields PA, Ping LH, Feinstone SM, Cromeans T, Jansen RW. Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination. J Virol. 1991 Apr;65(4):2056-65. PubMed PMID: 1705995; PubMed Central PMCID: PMC240056.
  2. Zhang H, Chao SF, Ping LH, Grace K, Clarke B, Lemon SM. An infectious cDNA clone of a cytopathic hepatitis A virus: genomic regions associated with rapid replication and cytopathic effect. Virology. 1995 Oct 1;212(2):686-97. PubMed PMID: 7571438.

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