The plasmid pHM175/18f.2 encodes for infectious molecular clone of cell culture-adapted, rapidly replicating, cytopathic human hepatitis A virus, genotype 1a, strain HM175/18f.
Hepatitis A is an infectious disease of the liver caused by the hepatitis A virus (HAV). It is usually spread by eating food or drinking water contaminated with infected feces. Symptoms are similar to influenza and include nausea, vomiting, diarrhea, jaundice, fever, and abdominal pain. Hepatitis A vaccine is routinely available and effective.
From the laboratory of Stanley M. Lemon, MD, University of North Carolina at Chapel Hill.
|Gene/insert name:||Hepatitis A virus genotype 1a, variant 18f|
|Accession ID:||GenBank: KP879216.1|
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||HindIII (destroyed)|
|Cloning Site 3':||SphI (destroyed)|
|Insert Size:||7531, including 3? poly(A) tract|
|Vector Backbone and Size:||pGEM3 (modified) (2844)|
|High or low copy:||High|
|Comments:||Linearize plasmid DNA with MluI for T7 transcript production.|
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