Anti-Zaire Ebola Virus VP35 Protein, C-Terminal [6C5] Antibody

This mouse IgG2a monoclonal antibody [6C5] was generated against DNA immunization followed by recombinant protein and is specific for the C-terminal portion of Zaire ebolavirus polymerase cofacter VP35 protein.

Highlights:

  • Reacts with the C-terminal portion of Zaire ebolavirus polymerase cofacter VP35 protein
  • Suitable for Western Blot, Immunoprecipitation and Immunofluorescence applications

Recombinant versions available at our sister company, Absolute Antibody:

Ebola virus (EBOV), or sometimes referred to as Zaire ebolavirus is one of the five identified Ebola virus species. Zaire ebolavirus is a virological taxon that comes from the genus, Ebolvarius, family Filoviridae and the order Mononegavirales. Ebola, often fatal has been shown to cause hemorrhagic fever in humans and other mammals including monkeys and chimpanzees. Polymerase cofactor VP35 acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. It prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3). This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1. VP35 also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.

From the laboratory of Christopher F. Basler, PhD, Icahn School of Medicine at Mount Sinai

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EMS702
Anti-Zaire Ebola Virus VP35 Protein, C-Terminal [6C5] Antibody
100ug In stock
Regular Price:$403.00
Specifications

Product Type: Antibody
Accession ID: AF086833.2 Ebola Mayinga, Zaire 1976
Antigen: Zaire ebolavirus polymerase cofactor VP35
Molecular Weight: 35 kDa
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: 6C5
Reactivity: Virus
Immunogen: DNA immunization followed by recombinant protein
Species Immunized: Mouse
Epitope: Within C-terminal portion of protein
Purification Method: Protein G Affinity
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1:1000), IP (1:500), IF
Storage: -20C
Shipped: Cold packs

Provider
From the laboratory of Christopher F. Basler, PhD, Icahn School of Medicine at Mount Sinai
References
  1. Olsen ME, Filone CM, Rozelle D, Mire CE, Agans KN, Hensley L, Connor JH.Polyamines and Hypusination Are Required for Ebolavirus Gene Expression and Replication. MBio. 2016 Jul 26;7(4). pii: e00882-16. View Article
  2. Takamatsu Y, Kolesnikova L, Becker S. Ebola virus proteins NP, VP35, and VP24 are essential and sufficient to mediate nucleocapsid transport. Proc Natl Acad Sci U S A. 2018 Jan 16. pii: 201712263. View Article
  3. Fang J, Pietzsch C, Ramanathan P, Santos RI, Ilinykh PA, Garcia-Blanco MA, Bukreyev A, Bradrick SS. Staufen1 Interacts with Multiple Components of the Ebola Virus Ribonucleoprotein and Enhances Viral RNA Synthesis. MBio. 2018 Oct 9;9(5). pii: e01771-18. View Article
  4. Miyake T, Farley CM, Neubauer BE, Beddow TP, Hoenen T, Engel DA. Ebola Virus Inclusion Body Formation and RNA Synthesis Are Controlled by a Novel Domain of Nucleoprotein Interacting with VP35. J Virol. 2020;94(16):e02100-19. Published 2020 Jul 30. View Article

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