E. coli Strain ALS1058

Escherichia coli cells cannot metabolize glucose.

From the laboratory of Mark A. Eiteman, PhD, University of Georgia.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA504
E. coli Strain ALS1058
1 vial Currently Unavailable
Regular Price:$310.00
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Specifications

Product Type: Bacteria
Name: ALS1058
Cell Type: Bacteria
Strain: ALS1058
Organism: Escherichia coli
Genotype: Hfr zbi::Tn10 poxB1 Δ(aceEF) rpsLpps-4 pfl-1 ldhA::Kan atpFH::Cam
Antibiotic Resistance: Kanamycin, chloramphenicol
Growth Conditions: TYA medium, 10g/L tryptone, 5g/L NaCl, 1g/L yeast extract, 1.36 g/L sodium acetate trihydrate Na(CH3COO)?3H2O, pH adjusted to 7.0, aerobic 37C
Format: Lyophilized culture
Storage: Room temperature as lyophilized culture
Shipped: Ambient temperature

Documentation

Protocol Notes

  1. Inject 1 mL of sterile DI water into vial and gently mix.
  2. Use small portions of vial (liquid) contents to inoculate a sterile, liquid culture to an initial OD of 0.03-0.1. For this liquid culture, use the specified growth medium with or without an antibiotic as appropriate.
  3. When culture has visibly grown in liquid medium, plate on solid (Agar) growth medium and incubate at 37C. Maintain strain.
Research
Strain Phenotype
ALS974 Accumulates lactic acid
ALS929 Accumulates pyruvic acid
ALS1392 Does not metabolize arabinose, glucose nor xylose
ALS1391 Does not metabolize arabinose nor xylose
ALS1371 Does not metabolize arabinose nor glucose
ALS1370 Does not metabolize xylose nor glucose
ALS1074 Does not metabolize xylose but can accumulate lactic acid from glucose
ALS1073 Does metabolize glucose but can accumulate lactic acid from xylose
ALS1060 Does not metabolize xylose nor glucose
ALS1059 Accumulates pyruvic acid
ALS1058 Does not metabolize glucose
ALS1048 Does not metabolize glucose
ALS1038 Does not metabolize xylose
ALS1054 Accumulates pyruvic acid
ALS1122 Does not metabolize xylose nor glucose
Provider
From the laboratory of Mark A. Eiteman, PhD, University of Georgia.
References
  1. Zhu Y, Eiteman MA, Altman R, Altman E. High glycolytic flux improves pyruvate production by a metabolically engineered Escherichia coli strain. Appl Environ Microbiol. 2008 Nov;74(21):6649-55.
  2. US Patent Number 8,551,758; PCT Publication number WO2008124162 A2. Pending applications in several jurisdictions – University of Georgia Research Foundation, Inc.

If you publish research with this product, please let us know so we can cite your paper.

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