Anti-RIG-I [1C3] Antibody
This mouse IgG1 monoclonal antibody was generated against recombinant human RIG-I caspase recruitment domain (CARD), amino acids 1-211, and is specific for human RIG-I.
Retinoic acid-inducible gene 1 protein (RIG-I), also known as DEAD-box protein 58, is coded for by the DDX58 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of innate immune responses. RIG-I is a cell receptor found in the cytoplasm of cells and functions as a pattern recognition receptor that is a sensor for viruses stimulating a type-I Interferon response through the mitochondrial protein IPS-1/MAVS.
From the laboratory of Adolfo Garcia-Sastre, PhD, Icahn School of Medicine at Mount Sinai.
This mouse IgG1 monoclonal antibody was generated against recombinant human RIG-I caspase recruitment domain (CARD), amino acids 1-211, and is specific for human RIG-I.
Retinoic acid-inducible gene 1 protein (RIG-I), also known as DEAD-box protein 58, is coded for by the DDX58 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of innate immune responses. RIG-I is a cell receptor found in the cytoplasm of cells and functions as a pattern recognition receptor that is a sensor for viruses stimulating a type-I Interferon response through the mitochondrial protein IPS-1/MAVS.
From the laboratory of Adolfo Garcia-Sastre, PhD, Icahn School of Medicine at Mount Sinai.
| Product Type: | Antibody |
| Name: | Anti RIG-I monoclonal antibody (1C3) |
| Accession ID: | O95786 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 1C3 |
| Specificity: | Human RIG-I |
| Immunogen: | Recombinant Human RIG-I caspase recruitment domain (CARD), amino acids 1-211 |
| Format: | Liquid |
| Purification Method: | Protein G purified |
| Tested Applications: | WB, IP (0.01 mg/mL cellular lysate) |
| Storage: | -20C |
| Shipped: | Cold packs |
| Western blot analysis from RIG-I pulldowns shows the efficiency of RIG-I immunoprecipitations. Adapted from: Baum et al. Preference of RIG-I for short viral RNA molecules in infected cells revealed by next-generation sequencing. Proc Natl Acad Sci, 107, 16303, 2010. |  |
- Baum et al. Preference of RIG-I for short viral RNA molecules ininfected cells revealed by next-generation sequencing. Proc Natl Acad Sci, 107, 16303, 2010.
- Nistal-Villan et al. Negative Role of RIG-I Serine 8 Phosphorylation in the Regulation of Interferon-beta Production. Journal of Biological Chemistry, 285, 20252, 2010.
- Durbin AF, Wang C, Marcotrigiano J, Gehrke L. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling. MBio. 2016 Sep 20;7(5). pii: e00833-16. View Article
- Sato S, Li K, Kameyama T, Hayashi T, Ishida Y, Murakami S, Watanabe T, Iijima S, Sakurai Y, Watashi K, Tsutsumi S, Sato Y, Akita H, Wakita T, Rice CM, Harashima H, Kohara M, Tanaka Y, Takaoka A. The RNA sensor RIG-I dually functions as an innate sensor and direct antiviral factor for hepatitis B virus. Immunity. 2015 Jan 20;42(1):123-32. View Article
- Durbin AF, Wang C, Marcotrigiano J, Gehrke L. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling. mBio. 2016;7(5):e00833-16. Published 2016 Sep 20. View Article
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