Mouse Neuroblastoma Cell Line (N2a[TRb1]-274 A+B Clone AB-2)

Neuro2a[TRb1]-274 Mouse neuroblastoma cell lines stably expressing human thyroid hormone receptor beta 1 with mutations in the thyroid hormone response element (TRE) of the Dnmt3a gene introduced by CRISPR/Cas9 genome editing.

From the laboratory of Robert J. Denver, PhD, University of Michigan.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EMU060
Mouse Neuroblastoma Cell Line (N2a[TRb1]-274 A+B Clone AB-2)
1 Vial In stock
Regular Price:$580.00
On Sale:
Specifications

Product Type: Cell Line
Name: N2a[TRb1-274A+B Clone AB-2]
Cell Type: Neuron
Morphology: Neuronal
Organism: Mouse
Source: Mouse neuroblastoma
Biosafety Level: BSL1
Growth Conditions: DMEM/F12 supplemented with hygromycin B (500 ug/mL) and 10% fetal bovine serum stripped of thyroid hormone.
Subculturing: Can be passaged with trypsin per standard protocols
Cryopreservation: DMEM/F12 growth medium with 10% DMSO
Comments: Mutation of the TRE in the Dnmt3a gene abolished the transcriptional response to thyroid hormone (T3).
Storage: LN2
Shipped: Dry Ice

Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
References
  1. Kyono, Y., Subramani, A., Ramadoss, P., Hollenberg, A.N., Bonett, R.M. and Denver, R.J. (2016) Liganded thyroid hormone receptors transactivate the DNA methyltransferase 3a gene in mouse neuronal cells. Endocrinology 157:3647-3657 doi: 10.1210/en.2015-1529 PMID:27387481

If you publish research with this product, please let us know so we can cite your paper.

Loading...