Anti-GW182/TNRC6A [4B6] Antibody
![Anti-GW182/TNRC6A [4B6] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1098_2001202001263410970.jpg "Anti-GW182/TNRC6A [4B6] Antibody")
This mouse IgG1 monoclonal antibody [4B6] was generated against recombinant protein and recognizes human and rabbit GW182.
Highlights:
- Reacts with human and rabbit GW182
- Suitable for Western Blot and Immunofluorscence applications
GW bodies (GWBs) function as storage centers and degradation sites for mRNAs. GW182 is a cytoplasmic marker protein for GWBs that bids to mRNA. GW182 autoantigen, also designated EMSY interactor protein, plays a role in the maintenance and stability of the GWB structures. The GW182 protein has been shown to interact with endogenous argonaute-2 (Ago2), which is also enriched in GWBs. The GW182 protein is found in patients with ataxia, Sjoegren’s syndrome (SS) and sensor neuropathy disease by developing autoantibodies against GWB proteins.
From the laboratory of Marvin J. Fritzler, MD, PhD, University of Calgary.
Part of The Investigator's Annexe program.
This mouse IgG1 monoclonal antibody [4B6] was generated against recombinant protein and recognizes human and rabbit GW182.
Highlights:
- Reacts with human and rabbit GW182
- Suitable for Western Blot and Immunofluorscence applications
GW bodies (GWBs) function as storage centers and degradation sites for mRNAs. GW182 is a cytoplasmic marker protein for GWBs that bids to mRNA. GW182 autoantigen, also designated EMSY interactor protein, plays a role in the maintenance and stability of the GWB structures. The GW182 protein has been shown to interact with endogenous argonaute-2 (Ago2), which is also enriched in GWBs. The GW182 protein is found in patients with ataxia, Sjoegren’s syndrome (SS) and sensor neuropathy disease by developing autoantibodies against GWB proteins.
From the laboratory of Marvin J. Fritzler, MD, PhD, University of Calgary.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Alternative Name(s): | Rinucleotide repeat containing 6A, GW1; GW182; TNRC6; CAGH26 |
| Accession ID: | NM_014494 |
| Antigen: | GW182 |
| Molecular Weight: | 182 kDa |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 4B6 |
| Reactivity: | Human and rabbit |
| Immunogen: | Recombinant protein |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | IF 1:10-1:50, WB Neat-1:10 |
| Storage: | -80C |
| Shipped: | Cold Packs |
- Jakymiw A, Lian S, Eystathioy T, Li S, Satoh M, Hamel JC, Fritzler MJ, Chan EK. Disruption of GW bodies impairs mammalian RNA interference. Nat Cell Biol. 2005 Dec;7(12):1267-74.
- Sen GL, Blau HM. Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies. Nat Cell Biol. 2005 Jun;7(6):633-6.
- Meister G, Landthaler M, Peters L, Chen PY, Urlaub H, Lührmann R, Tuschl T. Identification of novel argonaute-associated proteins. Curr Biol. 2005 Dec 6;15(23):2149-55.
- Moser JJ, Fritzler MJ. Cytoplasmic ribonucleoprotein (RNP) bodies and their relationship to GW/P bodies. Int J Biochem Cell Biol. 2010 Jun;42(6):828-43.
- Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME. TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5.
- Eystathioy T, Chan EK, Tenenbaum SA, Keene JD, Griffith K, Fritzler MJ. A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles. Mol Biol Cell. 2002 Apr;13(4):1338-51.
- Eystathioy T, Chan EK, Mahler M, Luft LM, Fritzler ML, Fritzler MJ. A pA binding protein GW182. Hybrid Hybridomics. 2003 Apr;22(2):79-86.
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Sharma NR, Majerciak V, Kruhlak MJ, et al. KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182. Nucleic Acids Res. 2019;47(17):9368-9385. View article
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