Anti-Rhamnogalacturonan-I [CCRC-M35] Antibody (supernatant)
![Anti-Rhamnogalacturonan I [CCRC-M35] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1030_2001202001263110290.jpg "Anti-Rhamnogalacturonan-I [CCRC-M35] Antibody (supernatant)")
This mouse IgM monoclonal (CCRC-M35) was generated against seed mucillage/MeBSA complex (Non-covalent) and belongs to Rhamnogalacturonan-I Backbone clade of antibodies and binds to the unsubstituted backbone of Rhamnogalacturonan-I.
Highlights:
- Reacts with the unsubstituted backbone of Rhamnogalacturonan-I
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of α-D-GalpA-(1,2)-α-L-Rhap-(1), and carry diverse side chains that are attached to the Rha residues and are composed largely of arabinosyl and/or galactosyl residues.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgM monoclonal (CCRC-M35) was generated against seed mucillage/MeBSA complex (Non-covalent) and belongs to Rhamnogalacturonan-I Backbone clade of antibodies and binds to the unsubstituted backbone of Rhamnogalacturonan-I.
Highlights:
- Reacts with the unsubstituted backbone of Rhamnogalacturonan-I
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of α-D-GalpA-(1,2)-α-L-Rhap-(1), and carry diverse side chains that are attached to the Rha residues and are composed largely of arabinosyl and/or galactosyl residues.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
| Product Type: | Antibody |
| Antigen: | Rhamnogalacturonan-I of Arabidopsis thaliana |
| Accession ID: | CCRC-M35 |
| Isotype: | IgM |
| Clonality: | Monoclonal |
| Clone Name: | CCRC-M35 |
| Reactivity: | Many plants |
| Immunogen: | Seed mucillage/MeBSA (Non-covalent complex) |
| Species Immunized: | Mouse |
| Epitope: | Rha-(1,4)-GalA-(1,2)-Rha-(1,4)-GalA-(1,2)-Rha-(1,4) |
| Buffer: | Cell culture supernatant, Use undiluted or at 1:10 dilution |
| Tested Applications: | ELISA, Immunolabeling, IF |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
CCRC-M35 binds to the backbone of rhamnogalacturonan-I and requires at least two unbranched disaccharide repeats for binding. CCRC-M35 does not bind to branched sections of the backbone and is not sensitive to the identity of the sugar at the non-reducing terminus.
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- Young, R.E., McFarlane, H.E., Hahn, M.G., Western, T.L., Haughn, G.W., Samuels, A.L. (2008) Analysis of the Golgi apparatus in Arabidopsis seed coat cells during polarized secretion of pectin-rich mucilage. Plant Cell 20:1623-1638.
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive tool kit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
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