Leslie B. Poole, PhD, Wake Forest School of Medicine

Leslie B. Poole, PhD
Leslie B. Poole, PhD

The Poole lab studies the structural and chemical bases for the enzymatic functions of AhpF and AhpC. These enzymes operate together to form the components of alkyl hydroperoxide reductase (AhpR), a crticial enzyme responsible for NAD(P)H-linked reduction of toxic organic hydroperoxides In bacteria such as Salmonella typhimurium and Escherichia coli.

Read Leslie's related blog post, Probing for Protein Sulfenic Acids.

The Investigator's Annexe Part of The Investigator's Annexe program.

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References

  1. Poole, L.B., Klomsiri, C., Knaggs, S.A., Furdui, C.M., Nelson, K.J., Thomas, M.J., Fetrow, J.S., Daniel, L.W. & King, S.B. Fluorescent and affinity-based tools to detect cysteine sulfenic acid formation in proteins. Bioconjug Chem 18, 2004-17 (2007). PMC2526167
  2. Klomsiri, C., Nelson, K.J., Bechtold, E., Soito, L., Johnson, L.C., Lowther, W.T., Ryu, S.E., King, S.B., Furdui, C.M. & Poole, L.B. Use of dimedone-based chemical probes for sulfenic acid detection: evaluation of conditions affecting probe incorporation into redox-sensitive proteins. Methods Enzymol 473, 77-94 (2010)
  3. Nelson, K.J., Klomsiri, C., Codreanu, S.G., Soito, L., Liebler, D.C., Rogers, L.C., Daniel, L.W. & Poole, L.B. Use of dimedone-based chemical probes for sulfenic acid detection; methods to visualize and identify labeled proteins. Methods Enzymol 473, 95-115 (2010).
  4. Kaplan, N., Urao, N., Furuta, E., Kim, S.J., Razvi, M., Nakamura, Y., McKinney, R.D., Poole, L.B., Fukai, T. & Ushio-Fukai, M. Localized cysteine sulfenic acid formation by vascular endothelial growth factor:role in endothelial cell migration and angiogenesis. Free Radic Res 45, 1124-35 (2011).
  5. Oshikawa, J., Urao, N., Kim, H.W., Kaplan, N., Razvi, M., McKinney, R., Poole, L.B., Fukai, T. & Ushio-Fukai, M. Extracellular SOD-derived H2O2 promotes VEGF signaling in caveolae/lipid rafts and post-ischemic angiogenesis in mice. PLoS One 5, e10189 (2010). PMC2858087
  6. Wani, R., Qian, J., Yin, L., Bechtold, E., King, S.B., Poole, L.B., Paek, E., Tsang, A.W. & Furdui, C.M. Isoform-specific regulation of Akt by PDGF-induced reactive oxygen species. Proc Natl Acad Sci U S A 108, 10550-5 (2011).