S. Bruce King, PhD, Wake Forest University

S. Bruce King, PhD
S. Bruce King, PhD

The King lab focuses on understanding the redox chemistry responsible for biological activity. To achieve these research goals, the lab uses a combination of synthetic and physical organic chemistry, biochemistry and biophysics to elucidate the identity, reactivity and effects of biological redox species. Much of their work centers on nitric oxide (NO) and the role it performs in biological systems. Specifically, they pursue the synthesis and evaluation of new organic compounds as HNO donors and the development of new organic-based methods of detection. As part of this study, they are attempting to better define the reactions of HNO with biological molecules, especially thiols and thiol-containing proteins and to understand biological pathways of HNO formation.

The Investigator's Annexe Part of The Investigator's Annexe program.

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References

  1. King SB, Nagasawa HT, Lester P. [22] Chemical approaches toward generation of nitroxyl. Methods in Enzymology. 1999. 301: 211-220.
  2. Shoman ME, Dumond JF, Isbell TS, Crawford JH, Brandon A, Honovar J, Vitturi DA, White CR, Patel RP, King SB. J. Med. Chem. 2011. 54(4):1059-1070.
  3. Sha X, Isbell TS, Patel RP, Day CS, King SB. JACS. 2006 128: 9687-9692.
  4. Reisz, JA, Zink CN, King SB. J. Am. Chem. Soc. 2011. 133: 11675-11685.
  5. Gao WD, Murray CI, Tian Y, Zhong X, DuMond JF, Shen X, Stanley BA, Foster DB, Wink DA, King SB, Van Eyk JE, Paolocci N. Nitroxyl-mediated disulfide bond formation between cardiac myofilament cysteines enhances contractile function. Circ Res. 2012 Sep 28;111(8):1002-11.
  6. Poole, L.B., Klomsiri, C., Knaggs, S.A., Furdui, C.M., Nelson, K.J., Thomas, M.J., Fetrow, J.S., Daniel, L.W. & King, S.B. Fluorescent and affinity-based tools to detect cysteine sulfenic acid formation in proteins. Bioconjug Chem 18, 2004-17 (2007). PMC2526167
  7. Klomsiri, C., Nelson, K.J., Bechtold, E., Soito, L., Johnson, L.C., Lowther, W.T., Ryu, S.E., King, S.B., Furdui, C.M. & Poole, L.B. Use of dimedone-based chemical probes for sulfenic acid detection: evaluation of conditions affecting probe incorporation into redox-sensitive proteins. Methods Enzymol 473, 77-94 (2010)
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