A549p0#1a is a human cell line derived from lung adenocarcinoma that lacks mitochondrial DNA, lack functional mitochondrial electron transport chain, and is auxotrophic for uridine and pyruvate.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
|Product Type:||Cell Line|
|Name:||A549p0#1a, A549p0#1a or control|
|Growth Conditions:||DMEM high glucose, 10% FBS, 1 mM pyruvate, 50 ug/ml uridine|
|Subculturing:||Grows slow. Split 1:3 twice a week, or as needed. Change medium 1-2 times a week. Do not grow to confluence.|
|Cryopreservation:||Growth medium+10% DMSO|
|Comments:||Do not allow for medium to acidify as it may lead to cell death|
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