HT-22rho-0 is a murine hippocampal neuronal cell line that is highly sensitive to glutamate and is used to study glutamate-induced toxicity in neuronal cells. Neuronal cells derive their ATP almost exclusively through mitochondrial oxidative phosphorylation, which is disrupted in HT-22 cells devoid of mtDNA (HT-22 rho-0 cells).
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
|Product Type:||Cell Line|
|Name:||HT-22 rho-0 or control|
|Cell Type:||Hippocampal neuronal cells|
|Growth Conditions:||DMEM high glucose, 10% FBS, 1 mM pyruvate, 50 ug/ml uridine|
|Subculturing:||Grows slow. Split 1:3 twice a week, or as needed. Change medium 1-2 times a week. Do not grow to confluence.|
|Cryopreservation:||Growth medium+10% DMSO|
|Comments:||Cells are sensitive to acidification of the medium.|
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