HT-22rho-0 Cells

HT-22rho-0 is a murine hippocampal neuronal cell line that is highly sensitive to glutamate and is used to study glutamate-induced toxicity in neuronal cells. Neuronal cells derive their ATP almost exclusively through mitochondrial oxidative phosphorylation, which is disrupted in HT-22 cells devoid of mtDNA (HT-22 rho-0 cells).

Highlights:

  • Useful in studies aimed at elucidating the role of the oxidative phosphorylation in neuronal function
  • From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

Catalog Number Product Size AVAILABILITY Price Qty
ESA111
HT-22 rho-0 Cell Line
1 Vial In stock
Regular Price:$399.00
ESA112
HT-22 Control Cell Line
1 Vial In stock
Regular Price:$399.00
Specifications

Product Type: Cell Line
Name: HT-22 rho-0 or control
Cell Type: Hippocampal neuronal cells
Organism: Mouse
Morphology: Epithelial
Source: Brain/hippocampus
Growth Conditions: DMEM high glucose, 10% FBS, 1 mM pyruvate, 50 ug/ml uridine
Subculturing: Grows slow. Split 1:3 twice a week, or as needed. Change medium 1-2 times a week. Do not grow to confluence.
Cryopreservation: Growth medium+10% DMSO
Comments: Cells are sensitive to acidification of the medium.
Storage: Liquid Nitrogen
Shipped: Dry Ice

Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
References
  1. Khozhukhar N, Spadafora D, Rodriguez Y, Alexeyev M. Elimination of Mitochondrial DNA from Mammalian Cells. Curr Protoc Cell Biol. 2018;78(1):20.11.1-20.11.14.

If you publish research with this product, please let us know so we can cite your paper.

Loading...