Arabinogalactan Protein (AGP) Antibodies
![Anti-Arabinogalactan-Protein (AGP) [LM14] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1046_2001202001263210450.jpg "Anti-Arabinogalactan-Protein (AGP) [LM14] Antibody")
These rat IgM monoclonal antibodies were generated against polysaccharide and recognize land plants arabinogalactan protein (AGP).
Highlights:
- Reacts with land plants AGP
- Suitable for Immunofluorescence and ELISA (Clone LM2 only) applications
Generated to rice arabinogalactan-proteins (AGPs). Recognizes a carbohydrate epitope containing Β-linked glucuronic acid. It can recognize AGPs in several species. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-Ο-methyl-Β-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-Ο-methyl-Β-D-GlcA. Arabinogalactans are plant glycans composed primarily of arabinosyl and/or galactosyl residues. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
From the laboratory of Paul Knox, PhD, University of Leeds.
These rat IgM monoclonal antibodies were generated against polysaccharide and recognize land plants arabinogalactan protein (AGP).
Highlights:
- Reacts with land plants AGP
- Suitable for Immunofluorescence and ELISA (Clone LM2 only) applications
Generated to rice arabinogalactan-proteins (AGPs). Recognizes a carbohydrate epitope containing Β-linked glucuronic acid. It can recognize AGPs in several species. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-Ο-methyl-Β-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-Ο-methyl-Β-D-GlcA. Arabinogalactans are plant glycans composed primarily of arabinosyl and/or galactosyl residues. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
From the laboratory of Paul Knox, PhD, University of Leeds.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Arabinogalactan-protein (AGP) |
| Isotype: | IgM (except JIM8, IgG2c) |
| Clonality: | Monoclonal |
| Clone Name: | Jim4, 8, 13, 15, 16 and LM2, 14, 30 |
| Reactivity: | Land Plants |
| Immunogen: | Polysaccharide |
| Species Immunized: | Rat |
| Epitope: | LM2 and LM14: Bind to Glucuronosyl residue of AGP, LM30: Binds to Arabinosyl residues of AGP, JIM16: Binds to Galactosyl residue of AGP, Others: unknown |
| Buffer: | Cell Culture Supernatant, 0.05% Sodium Azide |
| Tested Applications: | IF 1:10, ELISA 1:10 (LM2 only) |
| Storage: | -20C |
| Shipped: | Room Temperature |
- Smallwood M, Yates EA, Willats WGT, Martin H, Knox JP (1996) Immunochemical comparison of membrane-associated and secreted arabinogalactan-proteins in rice and carrot. Planta 198, 452-459
- Wilkinson et al. (2017) J. Cereal Science 74, 155-164.
- Stacey NJ, Roberts K, Knox JP. Patterns of expression of the JIM4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in Daucus carota L. Planta. 1990;180(2):285‐292.
- Yates EA, Valdor JF, Haslam SM, et al. Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology. 1996;6(2):131‐139.
- Pennell RI, Janniche L, Kjellbom P, Scofield GN, Peart JM, Roberts K. Developmental Regulation of a Plasma Membrane Arabinogalactan Protein Epitope in Oilseed Rape Flowers. Plant Cell. 1991;3(12):1317‐1326.
- Knox JP, Linstead PJ, Cooper JPC, Roberts K. Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J. 1991;1(3):317‐326.
- Moller I, Marcus SE, Haeger A, et al. High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles. Glycoconj J. 2008;25(1):37‐48.
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