Flagellar Membrane Glycoprotein 1B (FMG-1B) Antibodies

These mouse IgG1 monoclonal antibodies were raised against N-linked carbohydrate epitope on the membrane glycoprotein and recognize Flagellar membrane glycoprotein 1B (FMG-1B).

Highlights:

  • Reacts with FMG-1B
  • FMG-1B #8 binds to a carbohydrate epitope
  • FMG-1B #61 binds to an amino acid sequence in the core protein
  • Suitable for Immunocytochemistry, Immunofluorescence, Western Blot, Immunoprecipitation and Flow Cytometry applications

FMG-1B is the major membrane glycoprotein in the flagella of the bi-flagellate green alga, Chlamydomonas. FMG-1B is the flagellar component that mediates IFT motor-dependent force transduction at the flagellar surface.

From the laboratory of Robert A. Bloodgood, PhD, University of Virginia.

Catalog Number Product Size AVAILABILITY Price Qty
EG0044
Anti-Flagellar Membrane Glycoprotein 1B (FMG-1B) [Clone #8] Antibody
100ug 3-5 weeks
Regular Price:$299.00
EG0045
Anti-Flagellar Membrane Glycoprotein 1B (FMG-1B) [Clone #61] Antibody
100ug 3-5 weeks
Regular Price:$299.00
Specifications

Product Type: Antibody
Accession ID: AAO25118.2
Antigen: Flagellar membrane glycoprotein 1B/ FMG-1B
Molecular Weight: 350 kDa (apparent)
Isotype: IgG1
Clonality: Monoclonal
Clone Name: Clone #8 or Clone #61
Reactivity: Only reacts with Chlamydomonas flagellar membrane glycoprotein
Immunogen: Clone #8: N-linked carbohydrate epitope on the membrane glycoprotein; Clone #61: SDS-denatured protein taken from SDS-PAGE gel band.
Species Immunized: Mouse
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: Clone #8: WB (1:10,000), ICC (1:150), IF (1:200), IP, FC; Clone #61: WB (1:10,000)
Storage: -20C, Avoid freeze/thaw cycles and dilute in glycerol
Shipped: Cold Packs

Provider
From the laboratory of Robert A. Bloodgood, PhD, University of Virginia.

References
  1. Bloodgood RA, Woodward MP, Salomonsky NL. Redistribution and shedding of flagellar membrane glycoproteins visualized using an anti-carbohydrate monoclonal antibody and concanavalin A. J Cell Biol. 1986;102(5):1797-1812.

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