pJW231 and pJW232 plasmids offer an infections clone system that produce virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.
Zika virus (ZIKV; family Flaviviridae, genus Flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. This ZIKV reverse genetics system based on a 2015 isolate from Puerto Rico (strain PRVABC59). This infectious clone produces virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.
From the laboratories of Brian J. Geiss, PhD and Gregory D. Ebel, ScD, Colorado State University.
pJW231 (aka p1-ZIKV): T7 Promoter/Zika virus (strain PRVABC59) 5' end (1-3498)
pJW232 (aka p2-ZIKV): Zika virus (strain PRVABC59) 3' end (3,109-10,809) and HDV Ribozyme
|Grow in E. coli at 37 C:||DH5-alpha E. Coli or similar. Can grow at 37C.|
|Cloning Site 5':||
|Cloning Site 3':||
pJW231: 3834 bp
pJW232: 9458 bp
|Vector Backbone and Size:||pACYC177|
|High or low copy:||Low|
|Comments:||Using chloramphenicol amplification can increase plasmid yield.|
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