DBCO-PEG4-DBCO

DBCO-PEG4-DBCO is an azide-reactive homobisfunctional labeling reagent with enhanced water solubility that can be used to convert an azide functionality into a DBCO functionality or to cross-link two azido-tagged molecules.

Highlights:

• Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
• Chemoselective – azides and DBCO groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency
• Extended PEG4 Spacer – reduces aggregation, minimizes steric hindrance, and enhances solubility

DBCO-PEG4-DBCO reacts specifically and efficiently with an azide group (e.g., side chain of homoazidoalanine) to form a stable triazole moiety. When applied in excess, this reagent can efficiently convert azide-tagged peptides or biopolymers into DBCO tagged-peptides or biopolymers. When applied in stoichiometric amounts DBCO-PEG4-DBCO can serve as a homobisfunctional azide-to-azide crosslinker for covalently linking azido-modified peptides or biopolymers via stable triazole moiety. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.