Hyperforin and adhyperforin are extracted from the flowers and buds of Hypericum perforatum (St. John's Wort) utilizing the patented SuperFluids CXP technology [Castor, US Patent].
Hyperforin dicyclohexylammonium salt (Hyperforin DCHA) is the salt of Hyperforin, the primary active constituent responsible for the antidepressant and anxiolytic properties of extracts of St. John's Wort. It acts as a reuptake inhibitor of serotonin and other monoamines including norepinephrine, dopamine, and of GABA and glutamate by activating the transient receptor potential ion channel TRPC6. It also is an activator of the pregnane X receptor (PXR), which serves as a key regulator of CYP3A4 transcription, a member of the cytochrome (CYP) P450 enzyme system. Recent studies have found other neurological effects, effects on inflammation, as well as antibacterial, antitumoral and antiangiogenic effects.
|Product Type:||Small Molecule|
|Chemical Formula:||C35H52O4, C12H23N|
|Format:||White to off-white crystalline solid|
|Purity:||>99.5 % determined by HPLC; <0.1 % Adhyperforin by HPLC|
|Solubility:||Soluble in methanol and ethanol|
|Stability:||Samples stored at 5°C, 25°C, and 43°C for 60 days showed no decomposition as indicated by UV absorption or HPLC. Hyperforin DCHA stored at -20°C was stable after 2 years.|
|Storage:||5C, avoid exposure to direct sunlight|
Hyperforin DCHA HPLC Chromatogram.
Purification Notes: Initial silica chromatography separates oils and polar components from hyperforin and adhyperforin. Then C18 chromatography is used to separate hyperforin and adhyperforin. Hyperforin is in greater quantity and in the reversed phase system, hyperforin leads the adhyperforin. This means that the initial fractions will be hyperforin devoid of adhyperforin, and the later fractions will be adhyperforin contaminated with low levels of hyperforin. Hyperforin is then purified and stabilized by the formation of the dicyclohexylammonium salt.
If you publish research with this product, please let us know so we can cite your paper.