Recombinant Human Inactive Alpha Thrombin (S195A)
This S195A inactived human alpha thrombin protein was expressed recombinantly in E. Coli and purified to >95% (by SDS-PAGE).
Thrombin is a serine protease that possesses trypsin-like behavior in that it prefers to cleave its substrates after arginine and lysine residues. It elicits functional responses critical to blood homeostasis by interacting with diverse physiological substrates.
From the laboratory of Enrico Di Cera, MD, St. Louis University.
Part of The Investigator's Annexe program.
This S195A inactived human alpha thrombin protein was expressed recombinantly in E. Coli and purified to >95% (by SDS-PAGE).
Thrombin is a serine protease that possesses trypsin-like behavior in that it prefers to cleave its substrates after arginine and lysine residues. It elicits functional responses critical to blood homeostasis by interacting with diverse physiological substrates.
From the laboratory of Enrico Di Cera, MD, St. Louis University.
Part of The Investigator's Annexe program.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Protein |
| Name: | Human Alpha Thrombin (S195A) |
| Accession ID: | P00734, NM_000506 |
| Source: | E. coli |
| Molecular Weight: | 33.6 kDa |
| Amino Acid Sequence: | TFGSGEADCG LRPLFEKKSL EDKTERELLE SYIDGRIVEG SDAEIGMSPW QVMLFRKSPQ ELLCGASLIS DRWVLTAAHC LLYPPWDKNF TENDLLVRIG KHSRTRYERN IEKISMLEKI YIHPRYNWRE NLDRDIALMK LKKPVAFSDY IHPVCLPDRE TAASLLQAGY KGRVTGWGNL KETWTANVGK GQPSVLQVVN LPIVERPVCK DSTRIRITDN MFCAGYKPDE GKRGDACEGD (S>A)GGPFVMKSP FNNRWYQMGI VSWGEGCDRD GKYGFYTHVF RLKKWIQKVI DQFGE |
| Fusion Tag(s): | None |
| Purity: | >95% by SDS-PAGE |
| Buffer: | 300mM NaCl, 20mM Tris pH 7.4 |
| Concentration: | 0.1mg/mL |
| Activity: | S195A, Inactive |
| Comments: | The referenced sequences are processed to produce the alpha form of the enzyme. |
| Storage: | -80C |
| Shipped: | Dry ice |
Provider
From the laboratory of Enrico Di Cera, MD, St. Louis University.
References
- Marino F, Pelc LA, Vogt A, Gandhi PS, Di Cera E. Engineering thrombin for selective specificity toward Protein C and PAR1. Journal of Biological Chemistry, 285(25):19145-19152, (2010).
- Dang QD, Di Cera E. A simple activity assay for thrombin and hirudin. J Protein Chem. 1994 May;13(4):367-73.
- Estevez B, Kim K, Delaney MK, Stojanovic-Terpo A, Shen B, Ruan C, Cho J, Ruggeri ZM, Du X. Signaling-mediated cooperativity between glycoprotein Ib-IX and protease-activated receptors in thrombin-induced platelet activation. Blood. 2016 Feb 4;127(5):626-36. doi: 10.1182/blood-2015-04-638387. PubMed PMID: 26585954; PubMed Central PMCID: PMC4742550. View Article
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