FLAG-Frankenbody Expression Plasmids
Plasmids encoding anti-FLAG single-chain variable fragment (scFv) fused with mEGFP or HaloTag under CMV promoter.
Highlights:
- Encode mouse anti-FLAG- scFvs for H. sapiens (human) expression
- Engineered scFv binding the linear FLAG epitope with high affinity and specificity in vivo
- Light up in multiple colors FLAG-tagged nuclear, cytoplasmic, membrane, and mitochondrial proteins in diverse cell types
- Precisely quantify the expression of FLAG-tagged proteins in live cells
- Powerful tool for imaging protein dynamics in vivo
These plasmids genetically encode intracellular single-chain variable fragment that selectively binds the FLAG epitope (DYKDDDDK) with high affinity in living cells and organisms and can be used to quantify FLAG-tagged protein translation, localization, and dynamics.
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
Plasmids encoding anti-FLAG single-chain variable fragment (scFv) fused with mEGFP or HaloTag under CMV promoter.
Highlights:
- Encode mouse anti-FLAG- scFvs for H. sapiens (human) expression
- Engineered scFv binding the linear FLAG epitope with high affinity and specificity in vivo
- Light up in multiple colors FLAG-tagged nuclear, cytoplasmic, membrane, and mitochondrial proteins in diverse cell types
- Precisely quantify the expression of FLAG-tagged proteins in live cells
- Powerful tool for imaging protein dynamics in vivo
These plasmids genetically encode intracellular single-chain variable fragment that selectively binds the FLAG epitope (DYKDDDDK) with high affinity in living cells and organisms and can be used to quantify FLAG-tagged protein translation, localization, and dynamics.
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Plasmid |
| Gene/insert name: | anti-FLAG-scFv |
| Organism: | Mouse |
| Fusion Tag(s): | mEGFP or HaloTag |
| Tested Applications: | Resulting antibodies can be used for intracellular studies |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Kanamycin |
| Cloning Site 5': | EcoRI |
| Cloning Site 3': | NotI |
| Insert Size: | 1568bp (scFv-GFP) or 1742 bp (scFv-HaloTag) |
| Vector Backbone and Size: | pmCherry-C1 |
| High or low copy: | High |
| Promoter: | CMV driven |
| Storage: | -20C |
| Shipped: | Room temperature |
Provider
From the laboratory of Timothy J. Stasevich, PhD, Colorado State University.
References
- Liu Y, Zhao N, Kanemaki MT, Yamamoto Y, Sadamura Y, Ito Y, Tokunaga M, Stasevich TJ, Kimura H. Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Genes Cells. 2021 Nov;26(11):905-926. doi: 10.1111/gtc.12893. Epub 2021 Sep 20. PMID: 34465007; PMCID: PMC8893316.
- Cialek CA, Galindo G, Morisaki T, Zhao N, Montgomery TA, Stasevich TJ. Imaging translational control by Argonaute with single-molecule resolution in live cells. Nat Commun. 2022 Jun 10;13(1):3345. doi: 10.1038/s41467-022-30976-3. PMID: 35688806; PMCID: PMC9187665.
If you publish research with this product, please let us know so we can cite your paper.
