Histidyl-tRNA synthetase (HARS) 6x-His, N-Catalytic Domain

This N-terminal His-tagged 320-residue fragment of Histidyl-tRNA Synthetase from E. coli has been truncated immediately following motif 3.

Highlights:

  • Catalyzes both the specific aminoacylation of tRNA and pyrophosphate exchange
  • Shows no mischarging of noncognate tRNAs
  • Size exclusion chromatography showed that it is monomeric, indicating that the C-terminal domain is essential for maintaining the dimeric structure of the enzyme

Histidyl-tRNA synthetases attach amino acids to the 3' ends of their cognate transfer RNAs in a two-step reaction catalyzed by ATP. The accuracy of this reaction is crucial for the overall fidelity of protein synthesis. Defects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.

From the laboratory of Christopher S. Francklyn, PhD, University of Vermont.

The Investigator's AnnexePart of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EVM103
Histidyl-tRNA synthetase (HARS) 6x-His, N-Catalytic Domain, 50ug
50ug In stock
Price: $256.00
EVM112
Histidyl-tRNA synthetase (HARS) 6x-His, N-Catalytic Domain, 100ug
100ug In stock
Price: $410.00
EVM113
Histidyl-tRNA synthetase (HARS) 6x-His, N-Catalytic Domain, 250ug
250ug In stock
Price: $769.00
Specifications
Product Type: Protein
Name: N-Catalytic Domain Histidyl-tRNA synthetase w/6x His-tag
Accession ID: Derived from AAC75567, P60906
Source: E. coli
Molecular Weight: 36 kDa
Amino Acid Sequence: MRGSHHHHHHmakniqairgmndylpgetaiwqriegtlknvlgsygyseirlpiveqtplfkraigevtdvvekemytfedrngdsltlrpegtagcvragiehgllynqeqrlwyigpmfrherpqkgryrqfhqlgcevfglqgpdidaelimltarwwralgisehvtlelnsigslearanyrdalvafleqhkekldedckrrmytnplrvldsknpevqallndapalgdyldeesrehfaglckllesagiaytvnqrlvrgldyynrtvfewvtnslgsqgtvcaggrydglveqlggratpavgfamglerlvllvqavn
Fusion Tag(s): N-terminal 6x His-tag
Purity: > 95% by HisTrap
Buffer: 50 mM HEPES pH 7.5; 100 mM KCl; 10 mM MgCl2, 5 mM ?-mercaptoethanol, 40% glycerol
Concentration: 100 uM
Storage: Store at -20C
Shipped: Dry ice
Provider
From the laboratory of Christopher S. Francklyn, PhD, University of Vermont.
Comments

Protein has limited stability in aqueous buffers without glycerol

References
  1. Augustine, J., Francklyn, C., Design of an Active Fragment of a Class II Aminoacyl-tRNA Synthetase and Its Significance for Synthetase Evolution. Biochemistry, 36:12, 3473-3482, (1997).  

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