Pancreatic glucokinase (GK); Full-length Human

Glucokinase (GK; EC 2.7.1.2) is an enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Glucokinase occurs in cells in the liver, pancreas, gut, and brain of humans and most other vertebrates. In each of these organs it plays an important role in the regulation of carbohydrate metabolism by acting as a glucose sensor, triggering shifts in metabolism or cell function in response to rising or falling levels of glucose, such as occur after a meal or when fasting. Mutations of the gene for this enzyme can cause unusual forms of diabetes or hypoglycemia.

 

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Catalog Number Product Size AVAILABILITY Price Qty
FCA003
Pancreatic glucokinase (GK); Full-length Human, 25ug
25ug (1mg/mL) In stock
Price: $209.00
Specifications
Product Type: Protein
Source: E. Coli
Molecular Weight: 52.8 kDa
Format: Liquid
Purity: 80% (densitometry)
Buffer: 50 mM potassium phosphate pH-7.4, 50 mM sodium chloride, 0.5 mM ethylenediaminetetraaceticacid, and 2.5% glycerol.
Concentration: 1.0mg/mL
Comments: Human pancreatic glucokinase, (Accession No. NP_000153), aa 470 (full length) with C-terminal FLAG Tag, MW= 52.80 kDa, expressed in E.coli.
Storage: Stable >6 months -80C
Shipped: Dry ice
Comments

SDS-Page:

Specific Activity: 303 pmol/min/ug.  One unit is defined as the amount of enzyme that will convert 1 nmol of NADP to NADPH at 30 oC.  Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30 oC for 30 min.

Activation:  (±) Ro 28-0450 (100 uM) activated human pancreatic glucokinase 865% verses control measured at glucose [S]0.5.

References

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