Stable RAP Expression Plasmid
Plasmid containing the gene for Stable Receptor-Associated Protein (RAP).
Highlights:
- Used to express RAP
- Contains a stable RAP D3 domain that is resistant to both pH- and heat-induced denaturation
Stable Receptor-Associated Protein (RAP) is an antagonist of lipoprotein receptor-related protein 1 (LRP1). When RAP is administered IV post-stroke, it results in significant regaining of motor activity and a reduction in BBB leakage. RAP may also hold therapeutic promise in management of risk of bleeding in hemophiliac patients. The molecule binds to lipoprotein receptor-related protein 1 (LRP1) with high affinity and inhibits LRP1 function both in vitro and in vivo, suggesting that the novel RAP may be a useful tool in understanding lipoprotein metabolism, cell signaling, modulation of blood brain barrier integrity, and blood coagulation and fibrinolysis.
From the laboratory of Dudley K. Strickland, PhD, University of Maryland, Baltimore.
Plasmid containing the gene for Stable Receptor-Associated Protein (RAP).
Highlights:
- Used to express RAP
- Contains a stable RAP D3 domain that is resistant to both pH- and heat-induced denaturation
Stable Receptor-Associated Protein (RAP) is an antagonist of lipoprotein receptor-related protein 1 (LRP1). When RAP is administered IV post-stroke, it results in significant regaining of motor activity and a reduction in BBB leakage. RAP may also hold therapeutic promise in management of risk of bleeding in hemophiliac patients. The molecule binds to lipoprotein receptor-related protein 1 (LRP1) with high affinity and inhibits LRP1 function both in vitro and in vivo, suggesting that the novel RAP may be a useful tool in understanding lipoprotein metabolism, cell signaling, modulation of blood brain barrier integrity, and blood coagulation and fibrinolysis.
From the laboratory of Dudley K. Strickland, PhD, University of Maryland, Baltimore.
| Product Type: | Plasmid |
| Gene/insert name: | Stable Receptor Associated Protein (Stable RAP) |
| Accession ID: | P30533 |
| Organism: | Human |
| Antibiotic Resistance: | Ampicillin |
| Fusion Tag(s): | GST |
| Format: | Liquid |
| Grow in E. coli at 37 C: | Yes |
| Cloning Site 5': | BamH1 |
| Cloning Site 3': | EcoR1 |
| Insert Size: | 969 bp |
| Vector Backbone and Size: | 4948 bp |
| High or low copy: | High |
| Storage: | -20C |
| Shipped: | Room temperature |
- Prasad JM, Migliorini M, Galisteo R, Strickland DK. Generation of a Potent Low Density Lipoprotein Receptor-related Protein 1 (LRP1) Antagonist by Engineering a Stable Form of the Receptor-associated Protein (RAP) D3 Domain. J Biol Chem. 2015 Jul 10;290(28):17262-8.
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