Custom Nanobody Production

Custom Nanobody Production

Custom production of nanobodies or nanobody libraries to purified proteins.

Highlights:

  • Custom production of nanobodies or nanobody libraries to purified proteins (not peptides) derived from alpacas
  • Generated in alpacas utilizing purified proteins provided by the customer and produced recombinantly in E. coli
  • We provide 1mg of purified validated nanobody, complete sequence information, and the cDNA clone

Single domain antibodies (nanobodies) are increasingly becoming popular as reagents for research, for use as therapeutics, and as reagents for diagnostics. This is due, in large part, to their small size, stability, high affinity, high specificity, ease of manipulation, and ease of production.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EKY003
Custom Nanobody Production (Non-profit), 1mg
1mg nanobody; Complete sequence info; cDNA clone 6 months
Regular Price:$18,000.00
On Sale:
EKY004-FP
Custom Nanobody Production (For-profit), 1mg
1mg nanobody; Complete sequence info; cDNA clone 6 months
Regular Price:$21,000.00
On Sale:

Note: The list price is the starting cost. Final quoted price may vary depending on individual project requirements.
The next production run is set for November with antigens due here in late October. Once started, the process will take approximately 6 months.

Specifications

Product Type: Service
Name: Recombinant Nanobody Production Service
Source: The nanobodies are initially identified utilizing alpaca, and are then produced in and purified from E. coli
Purification Method: Affinity purification and molecular sieve chromatography
Method Used to Determine Concentration: OD280 or Bradford Assay
Buffer: PBS
Concentration: ~1mg/mL
Amount:

To produce a nanobody to your protein, we require:

  • 1-1.5mg of purified protein in PBS or HBS
  • Sample purity of >95% (as demonstrated by customer provided SDS-PAGE image) is required

Upon completion, we will provide:

  • 1 mg of purified and validated nanobody
  • Complete sequence information
  • Sequenced bacterial expression vector that can be used for further nanobody production
  • Typically we identify additional nanobodies for your protein, and these can be purchased for a nominal cost
Storage: -20C
Shipped: Cold Packs (Domestic, Overnight); Dry Ice (International)

Research
References
  1. Chow KM, Whiteheart SW, Smiley JR, Sharma S, Boaz K, Coleman MJ, Maynard A, Hersh LB, Vander Kooi CW. Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies. J Vis Exp. 2019 Jan 26;(143). doi: 10.3791/58471. PubMed PMID: 30741259.

If you publish research with this product, please let us know so we can cite your paper.