Multicolor GLUT5-Permeable Fluorescent Probes
Coumarin-based fluorescent sugar conjugates applicable for the analysis of fructose transport.
Highlights:
- Fructose uptake is orchestrated via free passage through the fructose-specific transporter GLUT5
- Probes cover a broad range of the fluorescence spectrum providing essential tools for the evaluation of fructose transport capacity in live cells
- 2,5-anhydro-D-mannitol-coumarin based GLUT5-specific probes - ManCous - amenable for the direct analysis of GLUT5 activity in cells
- The focused coumarin library conjugated to the 2,5-anhydro-D-mannitol (ManCous) includes fluorescent probes that emit at different parts of the fluorescence spectrum, while maintaining the same excitation
- As GLUT5 reporters, the probes allow for a visual discrimination between GLUT5-proficient and GLUT5-deficient cells
- The spectral versatility of ManCou probes allows for combination studies through mismatching fluorescence colors of different reporters, such as nuclear and membrane dyes
GLUT5 is a fructose transporter expressed on the apical border of enterocytes in the small intestine. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen.
From the laboratory of Marina Tanasova, PhD, Michigan Technological University.
Coumarin-based fluorescent sugar conjugates applicable for the analysis of fructose transport.
Highlights:
- Fructose uptake is orchestrated via free passage through the fructose-specific transporter GLUT5
- Probes cover a broad range of the fluorescence spectrum providing essential tools for the evaluation of fructose transport capacity in live cells
- 2,5-anhydro-D-mannitol-coumarin based GLUT5-specific probes - ManCous - amenable for the direct analysis of GLUT5 activity in cells
- The focused coumarin library conjugated to the 2,5-anhydro-D-mannitol (ManCous) includes fluorescent probes that emit at different parts of the fluorescence spectrum, while maintaining the same excitation
- As GLUT5 reporters, the probes allow for a visual discrimination between GLUT5-proficient and GLUT5-deficient cells
- The spectral versatility of ManCou probes allows for combination studies through mismatching fluorescence colors of different reporters, such as nuclear and membrane dyes
GLUT5 is a fructose transporter expressed on the apical border of enterocytes in the small intestine. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen.
From the laboratory of Marina Tanasova, PhD, Michigan Technological University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Small Molecule |
| Chemical Formula: |
C15H17NO6: ManCou1 C16H16F3NO6: ManCou3 C21H21NO6: ManCou4 C17H20N2O7: ManCou11 C22H22N2O7: ManCou12 C20H20N2O6 : ManCou14 |
| Molecular Weight: |
307.30: ManCou1 375.90: ManCou3 383.40: ManCou4 364.35: ManCou11 426.14: ManCou13 384.38: ManCou14 |
| Format: | Yellow Solid |
| Purity: | >95%, HPLC |
| Solubility: | DMSO |
| Spectral Information: | NMR, HRMS |
| Storage: | -20C |
| Shipped: | Room temperature |
Provider
From the laboratory of Marina Tanasova, PhD, Michigan Technological University.
References
- Begoyan VV, Weselinski JL, Xia S, Fedie J, Kannan S, Ferrier A, Rao S, Tanasova M. Multicolor GLUT5-permeable fluorescent probes for fructose transport analysis. Chem Commun (Camb). 2018 Apr 12;54(31):3855-3858.
- Kannan, S.; Begoyan, V. V.; Fedie, J. R.; Xia, S.; Weselinski, L. J.; Tanasova, M.; Rao, S., Metabolism-Driven High-Throughput Cancer Identification with GLUT5-Specific Molecular Probes. Biosensors (Basel) 2018, 8 (2), e39-e50.
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