Anti-Avian Leukosis Virus Subgroup J (ALV-J) gp85 [G2] Antibody

This IgG1k monoclonal antibody recognizes avian leukosis virus (AVL) -J envelope glycoprotein, gp85.

Highlights:

  • Reacts with AVL J envelope glycoprotein, gp85
  • Suitable for ELISA and Immunofluorescence applications

Avian leukosis virus (ALV) infects mainly chickens but can also infect pheasants, partridges and quail. It belongs to the Alpharetrovirus genus of the family Retroviridae. It is divided into subgroups A, B, C, D, E and J, depending on their viral envelope proteins which determine immune response and host range. The ALV envelope encases a capsid and a single stranded RNA genome.

From the laboratory at United States Department of Agriculture/USDA.

Catalog Number Product Size AVAILABILITY Price Qty
ECD013-FP
Anti-Avian Leukosis Virus Subgroup J (ALV-J) gp85 [G2] Antibody, 100ug
100ug 3-5 weeks
Price: $299.00

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Specifications
Product Type: Antibody
Antigen: AVL
Molecular Weight: 90 kDa
Isotype: IgG1k
Clonality: Monoclonal
Epitope: gp85
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Storage: -20C
Shipped: Cold Packs
Research
In an attempt to develop a specific diagnostic test for avian leukosis virus (ALV) subgroup J (ALV-J) strain Hc1, four monoclonal antibodies (MAbs), JE9, G2, 145, and J47, were generated that are specific for ALV-J envelope glycoprotein, gp85. Polymerase chain reaction (PCR) was used to amplify genomic pro-viral DNA of Avian Disease and Oncology Laboratory (ADOL)-Hc1 and ADOL-4817 envelope genes. Both open reading frames encoding glycoproteins gp85 and gp37 were cloned into baculoviruses. Abundant expression of gp85 and gp37 was detected in the recombinant viruses with specific antibody to Hc1 strain of the ALV-J. The expressed proteins were used for immunization of mice to produce hybridoma cell lines secreting MAbs specific to ALV-J envelope protein. A panel of MAbs was generated by fusing NS1 myeloma cells and spleen cells from mice immunized with the recombinant baculoviruses. With the use of an immunofluorescence assay, three MAbs (JE9, G2, 145) reacted with ALV-J but not with subgroups A, B, C, D, or E of ALV. MAb J47 reacted with all exogenous subgroups of ALV including A, B, C, D, and J but not with endogenous subgroup E viruses. Western blot analysis was performed with all four MAbs against recombinant baculovirus and Hc1-infected chicken embryo fibroblast (CEF) lysates. A major band with a molecular weight about 90 kD corresponding to the size of ALV-J envelope was consistently obtained. With these MAbs, we detected the Hc1 antigen in CEFs infected with several ALV-J viruses isolated in the United States and also in tissue sections from chickens infected with Hc1 strain of ALV-J. These MAbs will be useful reagents for the diagnosis of ALV-J infection because they recognize a common antigenic epitope in six isolates tested thus far.
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References
  1. Qin A, Lee LF, Fadly A, Hunt H, Cui Z. Development and characterization of monoclonal antibodies to subgroup J avian leukosis virus. Avian Dis. 2001 Oct-Dec;45(4):938-45. PubMed PMID: 11785897.

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