Mouse Fibroblast, Rieske Protein (UQCRFS1) Knock-Out Cell Line
Mouse fibroblast line lacking the Risk Iron Sulfur Protein from complex III of the respiratory chain (RIESKE protein).
Rieske proteins are iron-sulfur protein (ISP) components of cytochrome bc1 complexes and cytochrome b6f complexes and responsible for electron transfer in some biological systems. To generate the cell line, primary lung fibroblast culture was stablished from a knockin mouse homozygous for the floxed UQCRFS1 (exon 2) gene. UQCRFS1 encodes for the Rieske iron sulfur protein, subunit of complex III. Cells were immortalized by retroviral transduction with E6/E7 type 16 papiloma virus carrying G418 resistance. Clones were obtained from immortalization. One of the clones #8 was used to generate the RIESKE KO cells by transfecting with a plasmid expressing cre recombinase. Clones were obtained after hygromycine selection. This is KO clone is #8.5. Generation and characterization of cell line is described in PMID: 22106410. Mouse used to generate cell line had mixed genetic background (129 svj and C57BL6J).
From the laboratory of Carlos T. Moraes, PhD, University of Miami.
Mouse fibroblast line lacking the Risk Iron Sulfur Protein from complex III of the respiratory chain (RIESKE protein).
Rieske proteins are iron-sulfur protein (ISP) components of cytochrome bc1 complexes and cytochrome b6f complexes and responsible for electron transfer in some biological systems. To generate the cell line, primary lung fibroblast culture was stablished from a knockin mouse homozygous for the floxed UQCRFS1 (exon 2) gene. UQCRFS1 encodes for the Rieske iron sulfur protein, subunit of complex III. Cells were immortalized by retroviral transduction with E6/E7 type 16 papiloma virus carrying G418 resistance. Clones were obtained from immortalization. One of the clones #8 was used to generate the RIESKE KO cells by transfecting with a plasmid expressing cre recombinase. Clones were obtained after hygromycine selection. This is KO clone is #8.5. Generation and characterization of cell line is described in PMID: 22106410. Mouse used to generate cell line had mixed genetic background (129 svj and C57BL6J).
From the laboratory of Carlos T. Moraes, PhD, University of Miami.
| Product Type: | Cell Line |
| Cell Type: | Lung Fibroblast |
| Accession ID: | P47985 |
| Morphology: | Fibroblast |
| Source: | Lung |
| Organism: | Mouse |
| Biosafety Level: | BSL 1 |
| Subculturing: | Split when confluent |
| Growth Conditions: | High Glucose Dulbecco's modified Eagle's medium containing 10% fetal bovine serum, 1 mM pyruvate, 50 ug/ml uridine |
| Cryopreservation: | Growing media with 60% FBS and 10% DMSO |
| Comments: | E6/E7 immortalized fibroblasts |
| Storage: | LN2 |
| Shipped: | Dry Ice |
- Diaz F, Enríquez JA, Moraes CT. Cells lacking Rieske iron-sulfur protein have a reactive oxygen species-associated decrease in respiratory complexes I and IV. Mol Cell Biol. 2012 Jan;32(2):415-29. doi: 10.1128/MCB.06051-11. Epub 2011 Nov 21. PubMed PMID: 22106410; PubMed Central PMCID: PMC3255782.
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