Anti-Dengue-1, 2, 3 Virus Non-Structural Protein 1 (NS1) [2G1] Antibody
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This mouse IgG1 monoclonal antibody was raised against affinity-purified, detergent-generated monomeric non-structural protein 1 (rNS1) and recognizes dengue virus (DENV) nonstructural-1 (NS1) glycoprotein.
Highlights:
- Reacts with dengue-1 virus non-structural protein 1
- Strong reactivity to DENV 1, DENV 2, and DENV 3
- Unique epitope (25EVHTWTEQYKFQADSP39) allows for pairing with other mAbs for development of NS1 capture ELISA
- No cross-reactivity to NS1 of West Nile virus and Yellow fever virus in WB and indirect ELISA
- Suitable for ELISA and Western Blot applications
Dengue virus (DENV) is the cause of dengue fever. It is a mosquito-borne single positive-stranded RNA virus of the family Flaviviridae; genus Flavivirus. The virus genome is positive-sense single stranded RNA (ssRNA) that codes for three structural proteins (capsid protein C, membrane protein M, envelope protein E) and seven nonstructural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5). DENV nonstructural-1 (NS1) glycoprotein is useful for diagnosis of DENV infections in the first 8 days of illness with any of the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4). However, NS1 diagnostics are less sensitive for secondary DENV infections so the utility of NS1 diagnostics in dengue endemic countries where there is predominantly secondary infections is being questioned. Heat-mediated immunecomplex dissociation (ICD) prior to testing serum samples can significantly improve NS1 test sensitivity in secondary infections but requires monoclonal antibodies (MAbs) reactive to heat-denatured NS1. In order to incorporate a simple heat-mediated ICD step, a crucial step was to develop new MAbs with high affinity and specificity to heat-denatured DENV NS1 protein. This MAb allows for the incorporation of a heat dissociation step to improve the sensitivity of DENV NS1 antigen detection in secondary infections.
From the laboratory of Centers for Disease Control and Prevention.
This mouse IgG1 monoclonal antibody was raised against affinity-purified, detergent-generated monomeric non-structural protein 1 (rNS1) and recognizes dengue virus (DENV) nonstructural-1 (NS1) glycoprotein.
Highlights:
- Reacts with dengue-1 virus non-structural protein 1
- Strong reactivity to DENV 1, DENV 2, and DENV 3
- Unique epitope (25EVHTWTEQYKFQADSP39) allows for pairing with other mAbs for development of NS1 capture ELISA
- No cross-reactivity to NS1 of West Nile virus and Yellow fever virus in WB and indirect ELISA
- Suitable for ELISA and Western Blot applications
Dengue virus (DENV) is the cause of dengue fever. It is a mosquito-borne single positive-stranded RNA virus of the family Flaviviridae; genus Flavivirus. The virus genome is positive-sense single stranded RNA (ssRNA) that codes for three structural proteins (capsid protein C, membrane protein M, envelope protein E) and seven nonstructural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5). DENV nonstructural-1 (NS1) glycoprotein is useful for diagnosis of DENV infections in the first 8 days of illness with any of the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4). However, NS1 diagnostics are less sensitive for secondary DENV infections so the utility of NS1 diagnostics in dengue endemic countries where there is predominantly secondary infections is being questioned. Heat-mediated immunecomplex dissociation (ICD) prior to testing serum samples can significantly improve NS1 test sensitivity in secondary infections but requires monoclonal antibodies (MAbs) reactive to heat-denatured NS1. In order to incorporate a simple heat-mediated ICD step, a crucial step was to develop new MAbs with high affinity and specificity to heat-denatured DENV NS1 protein. This MAb allows for the incorporation of a heat dissociation step to improve the sensitivity of DENV NS1 antigen detection in secondary infections.
From the laboratory of Centers for Disease Control and Prevention.
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| Product Type: | Antibody |
| Antigen: | Dengue-1 virus non-structural protein 1 (NS1) |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 2G1 |
| Immunogen: | Affinity-purified, detergent-generated monomeric rNS2 |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | ELISA, WB (1:50,000 dilution of 1.67mg/mL) |
| Storage: | -20C |
| Shipped: | Cold Packs |
From the laboratory of Centers for Disease Control and Prevention.
- Gelanew T, Poole-Smith BK, Hunsperger E. Development and characterization of mouse monoclonal antibodies against monomeric dengue virus non-structural glycoprotein 1 (NS1). J Virol Methods. 2015 Sep 15;222:214-23.
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