pBG218 Recombinant Sindbis Virus-GFP Expression Plasmid

Recombinant plasmid pBG218 expresses Sindbis virus (SINV) expressing GFP during viral replication for easy visualization.

Highlights:

  • Plasmid can be transfected mammalian cells to produce infectious Sindbis virus
  • Expresses Green Fluorescent Protein (GFP) for easy visualization of infection

The entire Sindbis virus genome was inserted into the pcDNA3.1 Zeo+ expression vector such that the transcription start site of the CMV promoter overlaps with the first viral nucleotide of the viral genome. A Hepatitis D virus (HDV) ribozyme was added to the 3' end of the viral genomic 35bp polyA sequence. The gene for GFP was behind the 3' subgenomic promoter to allow expression of GFP protein during viral infection. Transfection of this construct into mammalian cells produces a self-replicating Sindbis virus genome that expresses GFP only when the virus genome is replicated in vitro or in vivo.

From the laboratory of Brian J. Geiss, PhD, Colorado State University.

Catalog Number Product Size AVAILABILITY Price Qty
ECS008
pBG218 Recombinant Sindbis Virus Replicon Plasmid, 250ng
Spotted on Filter Paper In stock
Price: $66.00
Specifications
Product Type: Plasmid
Gene/insert name: Sindbis virus replicon
Organism: Sindbis Virus Strain TE3'2J (AR339)
Antibiotic Resistance: Amp
Fusion Tag(s): eGFP
Grow in E. coli at 37 C: DH5-alpha or XL10-Gold E. coli
Cloning Site 5': MluI
Cloning Site 3': XhoI
Insert Size: 13,363 bp
Vector Backbone and Size: pcDNA3.1 Zeo+
High or low copy: High
Storage: Room Temperature (Dried), -20C (liquid)
Shipped: Room Temperature
Provider
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
References
  1. Geiss BJ, Shimonkevitz LH, Sackal CI, Olson KE. Recombination-ready Sindbis replicon expression vectors for transgene expression. Virol J. 2007 Oct 26;4:112.PubMed PMID: 17963504; PubMed Central PMCID: PMC2164957.

If you publish research with this product, please let us know so we can cite your paper.

 
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